2024-03-28T10:56:44Zhttp://buleria.unileon.es/oai/requestoai:buleria.unileon.es:10612/107352020-12-10T09:04:12Zcom_10612_17col_10612_18
BULERIA. Repositorio Institucional de la Universidad de León
author
Martínez Pastor, Felipe
author
Álvarez García, Mercedes
author
Guerra, Camino
author
Chamorro Álvarez, César Ángel
author
Anel López, Luis
author
Paz Cabello, Paulino de
author
Anel Rodríguez, Luis
author
Álvarez Rodríguez, Manuel
other
Biologia Celular
2019-05-12T23:52:49Z
2019-05-12T23:52:49Z
2019-05-13
Theriogenology, 2019, vol. 125
https://www.sciencedirect.com/science/article/pii/S0093691X18305739
http://hdl.handle.net/10612/10735
Germplasm banking is a key technology enabling the ex-situ conservation of wild species. However, cryopreservation protocols must be tested to assure the applicability of the banked material. The objective of this study was defining a range of parameters for the composition of a semen extender for Cantabrian chamois epididymal spermatozoa (post-mortem collection). The freezing extender was based in a TES-Tris-fructose buffer, modifying its composition in three experiments: Osmolality of the buffer (320, 380 or 430 mOsm/kg, 8% glycerol, 15% egg yolk), glycerol (4 or 8%, 430 mOsm/kg, 15% egg yolk), egg yolk (10 or 15%, 430 mOsm/kg, 4% glycerol). Sperm was extended at 100 mill. spermatozoa/ml, cooled at 5 °C and frozen at −20 °C/min. Sperm quality was assessed pre and post-thawing (CASA, HOS test, abnormal forms, cytoplasmic droplets, and viability and acrosomal damage by flow cytometry). Freezability was good overall, with total motility of 65.5% ± 2.4 initial and 55.8% ± 2.4 post-thawing. The extenders affected the post-thaw sperm quality marginally. Whereas osmolalities and glycerol concentrations seemed not to differ, 430 mOsm/kg and 4% glycerol might be preferred. Egg yolk concentrations only differed on sperm velocity (VCL: 84.0 ± 6.7 μm/s in 10% vs. 70.7 ± 6.2 μm/s in 15%, P < 0.05). Our results suggest a good cryotolerance of chamois epididymal spermatozoa, with a preferred extender composition of hyperosmotic buffer, glycerol in the 4% range and lower egg yolk (10% range) than other ruminants.
Veterinaria
Extender osmolality, glycerol and egg yolk on the cryopreservation of epididymal spermatozoa for gamete banking of the Cantabric Chamois (Rupicapra pyrenaica parva)
info:eu-repo/semantics/article
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URL
https://buleria.unileon.es/bitstream/10612/10735/1/101%20Extender%20osmolality%2c%20glycerol%20and%20egg%20yolk%20Theriogenology_125_0109_2019.pdf
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101 Extender osmolality, glycerol and egg yolk Theriogenology_125_0109_2019.pdf
URL
https://buleria.unileon.es/bitstream/10612/10735/3/101%20Extender%20osmolality%2c%20glycerol%20and%20egg%20yolk%20Theriogenology_125_0109_2019.pdf.txt
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101 Extender osmolality, glycerol and egg yolk Theriogenology_125_0109_2019.pdf.txt