2024-03-19T11:46:44Zhttp://buleria.unileon.es/oai/requestoai:buleria.unileon.es:10612/108352020-12-10T08:56:18Zcom_10612_17col_10612_18
BULERIA. Repositorio Institucional de la Universidad de León
author
Gallego Albiach, Víctor
author
Vilchez Olivencia, María Carmen
author
Sánchez Peñaranda, David
author
Pérez Igualada, Luz María
author
Herráez Ortega, María Paz
author
Asturiano Nemesio, Juan Francisco
author
Martínez Pastor, Felipe
other
Biologia Celular
2019-05-24T10:04:38Z
2019-05-24T10:04:38Z
2019-05-24
Reproduction, Fertility and Development, 2015, vol. 27, n. 3
http://www.publish.csiro.au/RD/RD13198
http://hdl.handle.net/10612/10835
There has been a marked reduction in natural stocks of eels (genus Anguilla) over the past 60 years, and the culture of eels is still based on the capture of very large quantities of juveniles. It is necessary to close the life cycle in captivity in order to ease the pressure on wild populations. The aims of the present study were to evaluate sperm subpopulations (through cluster analysis of computer-aided sperm analysis data) in the European eel (Anguilla anguilla) and to assess the effects of motility acquisition time after activation (i.e. at 30, 60 and 90 s), the thermal regimen (i.e. 10°C (T10) or 15°C (T15) and up to 20°C, or constant at 20°C (T20)) and hormonal treatments (i.e. human chorionic gonadotropin (hCG), recombinant (r) hCG or pregnant mare serum gonadotropin (PMSG)) on these subpopulations. In all cases, we obtained three subpopulations of spermatozoa: low velocity and linear (S1); high velocity with low linearity (S2); and high velocity and linear (S3; considered high quality). Total motility and S1 were affected by acquisition time; thus, 30 s is recommended as the standard time for motility acquisition. When eels were kept at 20°C (T20), motility data fitted quadratic models, with the highest motility and proportion of S3 between Weeks 8 and 12 after the first injection. Lower temperatures (T10, T15) delayed spermiation and the obtaining of high-quality spermatozoa (S3), but did not seem to alter the spermiation process (similar subpopulation pattern). Conversely, the hormonal treatments altered both the dynamics of the subpopulation pattern and the onset of spermiation (with PMSG delaying it). Total motility and the yield of S3 with the widely used hCG treatment varied throughout the spermiation period. However, using rhCG allowed us to obtain high-quality and constant motility for most of the study (Weeks 7–20), and the S3 yield was also higher overall (61.8 ± 1.3%; mean ± s.e.m.) and more stable over time than the other hormonal treatments (averaging 53.0 ± 1.4%). Using T20 and rhCG would be more economical and practical, allowing us to obtain a higher number of S3 spermatozoa over an extended time.
Veterinaria
Subpopulation pattern of eel spermatozoa is affected by post-activation time, hormonal treatment and the thermal regimen
info:eu-repo/semantics/article
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URL
https://buleria.unileon.es/bitstream/10612/10835/1/76%20Subpopulation%20pattern%20of%20eel%20spermatozoa%20ReprodFertilDev_027_0529_2015.pdf
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76 Subpopulation pattern of eel spermatozoa ReprodFertilDev_027_0529_2015.pdf
URL
https://buleria.unileon.es/bitstream/10612/10835/3/76%20Subpopulation%20pattern%20of%20eel%20spermatozoa%20ReprodFertilDev_027_0529_2015.pdf.txt
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76 Subpopulation pattern of eel spermatozoa ReprodFertilDev_027_0529_2015.pdf.txt