2024-03-29T15:44:16Zhttp://buleria.unileon.es/oai/requestoai:buleria.unileon.es:10612/134942024-03-04T12:35:59Zcom_10612_17col_10612_18
BULERIA. Repositorio Institucional de la Universidad de León
author
Cobo-Díaz, José F.
author
Alvarez-Molina, Adrián
author
Alexa, Elena A.
author
Walsh, Calum J.
author
Mencía-Ares, Oscar
author
Puente-Gómez, Paula
author
Likotrafiti, Eleni
author
Fernández-Gómez, Paula
author
Prieto, Bernardo
author
Crispie, Fiona
author
Ruiz, Lorena
author
González Raurich, María Montserrat
author
López, Mercedes
author
Prieto, Miguel
author
Cotter, Paul
author
Álvarez Ordóñez, Avelino
other
Microbiologia
2021-10-15T10:33:06Z
2021-10-15T10:33:06Z
http://hdl.handle.net/10612/13494
10.1186/s40168-021-01131-9
2049-2618
Background: The microorganisms that inhabit food processing environments (FPE) can strongly influence the
associated food quality and safety. In particular, the possibility that FPE may act as a reservoir of antibiotic-resistant
microorganisms, and a hotspot for the transmission of antibiotic resistance genes (ARGs) is a concern in meat
processing plants. Here, we monitor microbial succession and resistome dynamics relating to FPE through a
detailed analysis of a newly opened pork cutting plant over 1.5 years of activity.
Results: We identified a relatively restricted principal microbiota dominated by Pseudomonas during the first 2
months, while a higher taxonomic diversity, an increased representation of other taxa (e.g., Acinetobacter,
Psychrobacter), and a certain degree of microbiome specialization on different surfaces was recorded later on. An
increase in total abundance, alpha diversity, and β-dispersion of ARGs, which were predominantly assigned to
Acinetobacter and associated with resistance to certain antimicrobials frequently used on pig farms of the region,
was detected over time. Moreover, a sharp increase in the occurrence of extended-spectrum β-lactamase-
producing Enterobacteriaceae and vancomycin-resistant Enterococcaceae was observed when cutting activities
started. ARGs associated with resistance to β-lactams, tetracyclines, aminoglycosides, and sulphonamides frequently
co-occurred, and mobile genetic elements (i.e., plasmids, integrons) and lateral gene transfer events were mainly
detected at the later sampling times in drains.
Conclusions: The observations made suggest that pig carcasses were a source of resistant bacteria that then
colonized FPE and that drains, together with some food-contact surfaces, such as equipment and table surfaces,
represented a reservoir for the spread of ARGs in the meat processing facility.
Atribución-NoComercial 4.0 Internacional
Genética
Tecnología de los alimentos
Microbial colonization and resistome dynamics in food processing environments of a newly opened pork cutting industry during 1.5 years of activity
info:eu-repo/semantics/article
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URL
https://buleria.unileon.es/bitstream/10612/13494/1/Cobo-Diaz_2021_Microbiome.pdf
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https://buleria.unileon.es/bitstream/10612/13494/4/Cobo-Diaz_2021_Microbiome.pdf.txt
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