2024-03-28T21:52:06Zhttp://buleria.unileon.es/oai/requestoai:buleria.unileon.es:10612/64582020-12-10T09:00:46Zcom_10612_17col_10612_18
Frandoloso, Rafael
Martínez Martínez, Sonia
Rodríguez Ferri, Elías Fernando
Yubero-Delgado, Sheila
Rodríguez-Lázaro, D.
Hernández, M.
Gutiérrez Martín, César Bernardo
2017-07-26T10:42:10Z
2017-07-26T10:42:10Z
2017-07-26
Clinical and Developmental Immunology, 2013
http://hdl.handle.net/10612/6458
The expression of chemokines (CCL-2 and CXCL-8) and cytokines (IL-1𝛼, IL-1𝛽, IL-6, TNF-𝛼, and IL-10) was evaluated by RTqPCR
in colostrum-deprived pigs vaccinated and challenged with Haemophilus parasuis serovar 5. Two vaccines containing native
proteins with affinity to porcine transferrin (NPAPTim and NPAPTit) were tested, along with two control groups: one inoculated
with PBS instead of antigen (challenge group (CHG)), and another one nonimmunized and noninfected (blank group). The use of
NPAPTim and NPAPTit resulted in complete protection against H. parasuis (no clinical signs and/or lesions), and both vaccines
were capable of avoiding the expression of the proinflammatory molecules to levels similar to physiological values in blank group.
However, overexpression of all proinflammatory molecules was observed in CHG group, mainly in the target infection tissues
(brain, lungs, and spleen). High expression of CCL-2, CXCL-8, IL-1𝛼, IL-1𝛽, and IL-6 can be considered one of the characteristics
of H. parasuis infection by serovar 5
Sanidad animal
Haemophilus parasuis Subunit Vaccines Based on Native Proteins with Affinity to Porcine Transferrin Prevent the Expression of Proinflammatory Chemokines and Cytokines in Pigs
info:eu-repo/semantics/article