2024-03-28T08:05:43Zhttp://buleria.unileon.es/oai/requestoai:buleria.unileon.es:10612/105712020-12-10T09:01:27Zcom_10612_17col_10612_18
Rainbow trout surviving infections of viral haemorrhagic septicemia virus (vhsv) show lasting antibodies to recombinant g protein fragments
Gómez-Casado, E.
Fregeneda Grandes, Juan Miguel
Olesen, Niels Jørgen
Lorenzen, Niels
Estepa Pérez, Amparo
Coll Morales, Julio
Encinas, Paloma
Sanidad Animal
Facultad de Veterinaria
Inmunología
Sanidad animal
Veterinaria
Zoología
Truchas
Inmunoglobulinas
Serología
P. 929-935
Rainbow trout antibodies (Abs) binding to recombinant fragments (frgs) derived from the protein G of the
viral haemorrhagic septicemia virus (VHSV)-07.71 strain, could be detected by ELISA (frg-ELISA) in
sera from trout surviving laboratory-controlled infections. Abs were detected not only by using sera from
trout infected with the homologous VHSV isolate but also with the VHSV-DK-201433 heterologous
isolate, which had 13 amino acid changes. Sera from healthy trout and/or from trout surviving infectious
haematopoietic necrosis virus (IHNV) infection, were used to calculate cut-off absorbances to
differentiate negative from positive sera. Specific anti-VHSV Abs could then be detected by using any of
the following frgs: frg11 (56-110), frg15 (65-250), frg16 (252-450) or G21-465. While high correlations
were found among the ELISA values obtained with the different frgs, no correlations between any frg-
ELISA and complement-dependent 50 % plaque neutralization test (PNT) titres could be demonstrated.
Between 4 to 10 weeks after VHSV-infection, more trout sera were detected as positives by using
heterologous frg-ELISA rather than homologous PNT. Furthermore, the percentage of positive sera
detected by frg11-ELISA increased with time after infection to reach 100 %, while those detected by
complement-dependent PNT decreased to 29.4 %, thus confirming that the lack of neutralising Abs does
not mean the lack of any anti-VHSV Abs in survivor trout sera. Preliminary results with sera from field
samples suggest that further refinements of the frg-ELISA could allow detection of anti-VHSV trout Abs
in natural outbreaks caused by different heterologous VHSV isolates.The homologous frg-ELISA method
could be useful to follow G immunization attempts during vaccine development and/or to best
understand the fish Ab response during VHSV infections. The viral frgs approach might also be used with
other fish species and/or viruses.
SI
2011-03-15
2019-04-22T23:21:49Z
2019-04-22T23:21:49Z
2019-04-23
info:eu-repo/semantics/article
Fish and Shellfish Immunology, 2011, vol. 30, n. 3
https://doi.org/10.1016/j.fsi.2011.01.021
http://hdl.handle.net/10612/10571
eng
Elsevier