2024-03-29T04:44:21Zhttp://buleria.unileon.es/oai/requestoai:buleria.unileon.es:10612/47062020-12-10T09:02:38Zcom_10612_17col_10612_18
Systemic and mammary gland disposition of enrofloxacin in healthy sheep following intramammary administration
López, Cristina
García, Juan J.
Sierra, Matilde
Diez, M. José
Pérez
Sahagún, Ana M.
Fernández, Nélida
Farmacologia
Facultad de Veterinaria
Farmacología
Sanidad animal
Veterinaria
Zoología
Enrofloxacin
Sheep
Intramammary
Systemic
Glandular tissue
Disposition
P. 88-94
Background: Mastitis is one of the most important diseases affecting dairy sheep. Antimicrobial drugs are often
administered directly through teat to treat or prevent this disease, but data on drug distribution within glandular
tissue are scarce and it cannot be estimated from concentrations in milk. Thus, the aim of this study was to
investigate systemic and mammary gland distribution of enrofloxacin after intramammary administration. The
drug was administered to 6 healthy lactating Assaf sheep with an injector containing an enrofloxacin preparation
(1 g drug/5 g ointment). Blood samples were collected at 0, 30, 60, 90, 120, 150 and 180 min. Animals were then
sedated and sacrificed, and glandular tissue samples were obtained from treated udders at 2, 4, 6 and 8 cm height.
Enrofloxacin concentrations were measured in plasma and tissue samples by UV high-performed liquid chromatography.
Results: Mean enrofloxacin plasma concentrations were below 0.5 μg/mL. Mean tissue concentrations decreased in
mammary gland with vertical distance from the teat, ranging from 356.6 μg/g at 2 cm to 95.60 μg/g at the base of the
udder. Glandular tissue concentrations best fitted to a decreasing monoexponential model, and showed a good
correlation with an ex vivo model previously developed.
Conclusions: Enrofloxacin concentrations were effective in the entire glandular tissue against the main pathogens
causing mastitis in sheep. These results suggest that this drug may be suitable to treat mastitis in sheep by
intramammary administration.
SI
2015-05-15
2015-10-21T14:18:16Z
2015-10-21T14:18:16Z
2015-10-21
info:eu-repo/semantics/article
BMC veterinary research, 2015, n. 11
http://hdl.handle.net/10612/4706
spa
Biomed central