2024-03-28T14:15:44Zhttp://buleria.unileon.es/oai/requestoai:buleria.unileon.es:10612/106972020-12-10T09:02:27Zcom_10612_17col_10612_18
Refrigerated storage of ram sperm in presence of Trolox and GSH antioxidants: Effect of temperature, extender and storage time
Mata Campuzano, María
Álvarez Rodríguez, Manuel
Tamayo Canul, Julio Renan
López Urueña, Elena
Paz Cabello, Paulino de
Anel Rodríguez, Luis
Martínez Pastor, Felipe
Álvarez García, Mercedes
Biologia Celular
Veterinaria
Antioxidants have a potential to improve the quality and fertility of refrigerated-stored ram semen. Reduced glutathione (GSH) and Trolox (0.2, 1 and 5 mM) were evaluated in ram semen preserved at 15 and 5 °C up to 48 and 96 h, respectively. Extenders were also evaluated (15 °C: Tris-citrate-fructose, TCF, without lipids, and TES-Tris-fructose 10% egg yolk, TTF-EY; 5 °C: TTF-EY and 3.5% soybean lecithin, TTF-SL; INRA96 at both temperatures). Storage at 5 °C resulted in poorer quality than 15 °C up to 48 h, while allowing acceptable quality at 96 h. Antioxidants had few effects on sperm quality, with use of Trolox resulting in reduced motility and viability in TCF. Storage at 15 °C in the TCF extender resulted in decreased motility, viability and mitochondrial activity compared with use of TTF-EY. Sperm quality when storage was at 5 °C was similar, but storage in TTF-SL resulted in decreased motility and mitochondrial activity. Acrosomal status was only slightly affected by extender and antioxidant. Mitochondrial activity was improved by antioxidants in TTF-SL, and GSH at 5 mM when semen was stored at 5 °C in TTF-EY. A preliminary artificial insemination trial indicated that supplementation with GSH has the potential for improving lambing (P < 0.1). In conclusion, use of antioxidants resulted in lesser effects than extender composition or storage time on quality of ram semen. Use of Trolox negatively impacted sperm quality and GSH had some positive impacts. The use of soybean lecithin requires further research to assess its impact on mitochondria.
2019-05-12T23:11:35Z
2019-05-12T23:11:35Z
2019-05-12T23:11:35Z
2019-05-13
info:eu-repo/semantics/article
Animal Reproduction Science, 2014, vol. 151, n. 3–4
https://www.sciencedirect.com/science/article/pii/S0378432014003042
http://hdl.handle.net/10612/10697
Elsevier