2024-03-28T10:31:47Zhttp://buleria.unileon.es/oai/requestoai:buleria.unileon.es:10612/80192020-12-10T09:02:03Zcom_10612_17col_10612_18
Antibody response of brown trout Salmo trutta injected with pathogenic Saprolegnia parasitica antigenic extracts
Fregeneda Grandes, Juan Miguel
Rodríguez Cadenas, Fausto
Carbajal González, María Teresa
Aller Gancedo, José Miguel
Sanidad Animal
Inmunología
Sanidad animal
Veterinaria
Zoología
Brown trout Salmo trutta injected with antigenic extracts from a pathogenic isolate of
Saprolegnia parasitica developed specific antibodies that were detected by enzyme-linked immunosorbent
assay (ELISA), immunofluorescence (IF) and Western blotting (WB), but not by immunodiffusion
(ID). Three groups of five 2 yr old brown trout were injected intraperitoneally with 3 different
antigenic extracts: small hyphal fragments (HF) and soluble extracts from sonicated mycelia
grown in medium with or without β-sytosterol (SEB and SE, respectively). In the 2 groups injected
with SE and SEB, antibodies were found in 66.7% of the serum samples by ELISA, 54.5% by IF and
48.5% by WB. In the group injected with HF, only 1 trout survived the experiment, and in this fish
only 1 sample was positive by ELISA. The results obtained by ELISA and IF were similar and show
that there is cross-reaction between the antigens used. By WB, the proteins most frequently recognised
were 2 proteins of 25 and 29 kDa. No significant differences were found in the groups injected
with SE or SEB.
2018-05-27T20:54:24Z
2018-05-27T20:54:24Z
2018-05-27T20:54:24Z
2018-05-27
info:eu-repo/semantics/article
Diseases of aquatic organisms, 2007, vol. 74, n. 2
http://hdl.handle.net/10612/8019
info:eu-repo/semantics/openAccess
Inter-Research