2024-03-29T06:11:22Zhttp://buleria.unileon.es/oai/requestoai:buleria.unileon.es:10612/107192020-12-10T09:02:32Zcom_10612_17col_10612_18
Outlining adequate protocols for Lidia bull epididymal storage and sperm cryopreservation: use of glycerol, dimethylformamide and N-acetylcysteine
Matilla Pinto, Elvira
González Fernández, Lauro
Martínez Pastor, Felipe
Hernández Rollán, Nuria
Tobajas Vega, Carolina
Calle Guisado, Violeta
Mijares Gordún, José
Sánchez Margallo, Francisco Miguel
Álvarez Miguel, Ignacio Santiago
Macías García, Beatriz
Biologia Celular
Veterinaria
P. 1-10
The Lidia bovine breed is an important hallmark of the Spanish cattle industry. Bulls are selected based upon aggressiveness and epididymal sperm cryopreservation is the way to obtain and store their genetics. There are not specifically designed protocols yet to perform Lidia bull sperm cryopreservation. The present study aimed to determine if a tris-fructose-citrate-egg yolk (20% v/v; TFY) extender supplemented with 7% glycerol (TFY1) or 3.5% glycerol plus 3.5% dimethylformamide (DMF; TFY2) are suitable media for cryopreservation of epididymal Lidia bull sperm. Moreover, the effect of N-acetylcysteine (NAC), a potent antioxidant, was evaluated. The epididymis were stored at 4°C for 24, 48, 72 or 96 h, and both freezing media were tested as such or supplemented with 1 or 2.5 mM of NAC. Our data demonstrated that post-thaw viability was well maintained (TFY1: 50.8% ± 1.9 at 24 h and 52.4% ± 0.8 at 96 h and TFY2: 52.6% ± 1.6 at 24 h and 56.1% ± 1.8 at 96 h; mean % ± SEM; p>0.05) as also were total and progressive sperm motility, high mitochondrial membrane potential, ROS production, DNA status and acrosomal intactness of Lidia bull sperm up to 96 h of epididymal storage, all extender variations being similar (p>0.05). In conclusion, the use of TFY medium supplemented either with 7% glycerol alone or the combination of 3.5% glycerol and 3.5% DMF were equally safe choices for epididymal Lidia bull sperm cryopreservation, and NAC addition did not significantly improve sperm post-thaw quality.
2019-05-12T23:42:20Z
2019-05-12T23:42:20Z
2019-05-13
info:eu-repo/semantics/article
Spanish Journal of Agricultural Research, 2017, vol. 15, n. 3
http://revistas.inia.es/index.php/sjar/article/view/11463
http://hdl.handle.net/10612/10719
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria