2024-03-28T15:20:54Zhttp://buleria.unileon.es/oai/requestoai:buleria.unileon.es:10612/102222020-12-10T09:01:43Zcom_10612_17col_10612_18
Facultad de Ciencias Biologicas y Ambientales
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500
Domínguez Rebolledo, Álvaro Efrén
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500
Fernández Santos, María Rocío
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500
Bisbal Vigo, Alfonso
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500
Ros Santaella, José Luis
bec98004-4d84-48b8-bd5d-d58655c4d87b
500
Ramón Fernández, Manuel
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500
Carmona Delgado, Manuel
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500
Martínez Pastor, Felipe
700
500
0000-0003-2987-4302
Garde López-Brea, Julián
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Biologia Celular
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500
2010-04-15
2019-04-14T23:24:06Z
2019-04-14T23:24:06Z
2019-04-15
Reproduction, Fertility and Development, 2010, vol. 22, n. 5
http://www.publish.csiro.au/rd/rd09197
http://hdl.handle.net/10612/10222
P. 856-870
Antioxidants could improve sperm media, extending the viability of spermatozoa and protecting their DNA. The protective ability of lipoic acid, melatonin, Trolox and crocin was tested on red deer spermatozoa incubated at 37°C. Cryopreserved spermatozoa were thawed and incubated with 1 mM or 0.1 mM of each antioxidant, with or without oxidative stress (100 μM Fe2+). Motility (CASA), viability, mitochondrial membrane potential and acrosomal status were assessed. Lipoperoxidation (malondialdehyde production), intracellular reactive oxygen species (ROS) and DNA status (TUNEL) were checked at 4 h. Incubation alone increased ROS and decreased motility. Oxidative stress intensified these effects, increasing lipoperoxidation and DNA damage. Lipoic acid had little protective effect, whereas 1 mM melatonin showed limited protection. Trolox lowered ROS and lipoperoxidation both in oxidised and non-oxidised samples. In oxidised samples, Trolox prevented DNA and acrosomal damage, and ameliorated motility. Crocin at 1 mM showed similar results to Trolox, but noticeably stimulated motility and had no effect on lipoperoxidation. In a second experiment, a broader range of crocin and melatonin concentrations were tested, confirming the effects of crocin (positive effects noticeable at 0.5–0.75 mM), but showing an increase in lipoperoxidation at 2 mM. Melatonin was increasingly effective at 2.5 and 5 mM (ROS, lipoperoxidation and DNA status). Crocin seems a promising new antioxidant, but its particular effects on sperm physiology must be further studied, especially the consequences of motility stimulation and confirming its effect on lipoperoxidation. Melatonin might be useful at relatively high concentrations, compared to Trolox.
SI
eng
CSIRO
Veterinaria
Crocin
Lipoic acid
Melatonin
Trolox
Improving the effect of incubation and oxidative stress on thawed spermatozoa from red deer by using different antioxidant treatments
info:eu-repo/semantics/article
THUMBNAIL
38 Improving the effect of incubation and oxidative stress ReprodFertilDev_022_0856_2010.pdf.jpg
38 Improving the effect of incubation and oxidative stress ReprodFertilDev_022_0856_2010.pdf.jpg
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38 Improving the effect of incubation and oxidative stress ReprodFertilDev_022_0856_2010.pdf.txt
38 Improving the effect of incubation and oxidative stress ReprodFertilDev_022_0856_2010.pdf.txt
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https://buleria.unileon.es/bitstream/10612/10222/3/38%20Improving%20the%20effect%20of%20incubation%20and%20oxidative%20stress%20ReprodFertilDev_022_0856_2010.pdf.txt
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38 Improving the effect of incubation and oxidative stress ReprodFertilDev_022_0856_2010.pdf
38 Improving the effect of incubation and oxidative stress ReprodFertilDev_022_0856_2010.pdf
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10612/10222
oai:buleria.unileon.es:10612/10222
2020-12-10 10:01:43.333
BULERIA. Repositorio Institucional de la Universidad de León
buleria@unileon.es
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