2024-03-28T11:41:36Zhttp://buleria.unileon.es/oai/requestoai:buleria.unileon.es:10612/106072020-12-10T09:01:20Zcom_10612_17col_10612_18
Facultad de Ciencias Biologicas y Ambientales
86f509c6-225f-4dc5-9dda-faf034f20a8d
500
Martínez Pastor, Felipe
700
500
0000-0003-2987-4302
García Macías, Vanesa
6c534982-aaab-4918-8f5f-62dc4aebb35c
500
Álvarez García, Mercedes
63
500
0000-0002-1185-2466
Chamorro Álvarez, César Ángel
226
500
0000-0001-6331-6916
Herráez Ortega, María Paz
569
500
0000-0002-0028-3616
Paz Cabello, Paulino de
803
500
0000-0003-2531-595X
Anel Rodríguez, Luis
86
500
0000-0003-2423-4031
Biologia Celular
00bf73ac-0b62-4731-8db6-c30ab33fc011
500
2006-02
2019-04-23T00:06:37Z
2019-04-23T00:06:37Z
2019-04-23
Theriogenology, 2006, vol. 65, n. 3
https://www.sciencedirect.com/science/article/pii/S0093691X0500230X
http://hdl.handle.net/10612/10607
P. 471-485
We have compared two methods for salvaging epididymal sperm from post-mortem samples from Iberian red deer. Of each pair of testicles (29 samples), one cauda epididymis was processed by means of cuts (sperm was immediately diluted with extender) and the other was detached from the corpus and flushed from the vas deferens with 1 mL of extender. Sperm was processed for cryopreservation, and analyzed just after recovery, pre-freezing and post-thawing. Total spermatozoa recovered, contamination (concentration of epididymal cells and red blood cells (RBCs)) and quality (motility by CASA, and acrosomal status, viability and mitochondrial status by flow cytometry) were used to compare both methods. The number of recovered spermatozoa was similar for both methods. Contamination was higher for the cuts method, but when considering the final dilution before freezing, only RBCs concentration was significantly higher. Motility was similar just after extraction, but higher for both pre-frozen and post-thawed flushed sperm. Pre-freezing acrosomal status (P < 0.05) and viability (P < 0.1) were better for flushing; however post-thawing results were similar for the two methods. A clustering analysis using CASA data showed that the subpopulation pattern of motile sperm was different depending on the method, being better for flushing. With regard to yield, lower contamination (especially RBCs) and, in general, better quality results, flushing seems to be a more recommendable method for post-mortem sperm recovery. The cuts method may be more practical on certain occasions, but care must be taken in order to achieve rapid extension of the sample and to avoid contamination in order to improve sample condition.
SI
eng
Elsevier
Veterinaria
Red deer
Post-mortem recovery
Contamination
Epididymal sperm
Method comparison
Comparison of two methods for obtaining spermatozoa from the cauda epididymis of Iberian red deer
info:eu-repo/semantics/article
THUMBNAIL
11 Comparison of two methods Theriogenology_065_0471_2006.pdf.jpg
11 Comparison of two methods Theriogenology_065_0471_2006.pdf.jpg
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11 Comparison of two methods Theriogenology_065_0471_2006.pdf.txt
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11 Comparison of two methods Theriogenology_065_0471_2006.pdf
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10612/10607
oai:buleria.unileon.es:10612/10607
2020-12-10 10:01:20.13
BULERIA. Repositorio Institucional de la Universidad de León
buleria@unileon.es
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