2024-03-28T20:29:31Zhttp://buleria.unileon.es/oai/requestoai:buleria.unileon.es:10612/90242020-12-10T09:00:28Zcom_10612_17col_10612_18
Facultad de Veterinaria
005f7248-275c-46b3-9219-965b78c9cc04
500
Fregeneda Grandes, Juan Miguel
386
500
0000-0002-2165-1350
Olesen, Niels Jørgen
8ed95215-e26c-4b32-b7da-64b4cd8b6406
500
Sanidad Animal
6a4dac48-8601-4df8-8039-194ca2c17a76
500
2007-02-28
2018-11-08T23:50:15Z
2018-11-08T23:50:15Z
2018-11-09
Diseases of aquatic organisms, 2007, vol. 74, n. 2
http://hdl.handle.net/10612/9024
P. 151-158
Three serological tests, enzyme linked immunosorbent assay (ELISA), 50% plaque neutralisation
test (50%PNT) and Western blotting (WB), were used to detect antibodies against viral
haemorrhagic septicaemia virus (VHSV) in 50 rainbow trout broodstock from a rainbow trout farm
endemically infected with VHS but with no clinical signs of infection. When the sera were examined
by 50%PNT using the VHSV reference isolate DK-F1 or the heat attenuated DK-F25 mutant strain,
no neutralizing antibodies were found. In contrast, when one of the virus isolates from the farm
(homologous virus) was used in the 50%PNT, 90% of the fish were found to be positive. By examining
a panel of different VHSV isolates in 50%PNT, it was demonstrated that the virus isolate used as
test antigen could significantly affect the sensitivity and titre determination in 50%PNT for detection
of rainbow trout antibodies against VHSV. When the sera were examined for the presence of VHSV
antibodies by ELISA or WB, 61% were found to be positive. When conducting WB analysis, the viral
glycoprotein was the protein most frequently recognized, followed by the viral nucleoprotein.
SI
eng
Inter-Research
Inmunología
Sanidad animal
Veterinaria
Zoología
Truchas
Serología
Inmunoglobulinas
Detection of rainbow trout antibodies against viral haemorrhagic septicaemia virus (VHSV) by neutralisation test is highly dependent on the virus isolate used
info:eu-repo/semantics/article
THUMBNAIL
DAO 74, 151-158, 2007 (antibodies against VHSV).pdf.jpg
DAO 74, 151-158, 2007 (antibodies against VHSV).pdf.jpg
IM Thumbnail
image/jpeg
4560
https://buleria.unileon.es/bitstream/10612/9024/3/DAO%2074%2c%20151-158%2c%202007%20%28antibodies%20against%20VHSV%29.pdf.jpg
bf2d38fa8fc4143db3c67d6560bb67c5
MD5
3
ORIGINAL
DAO 74, 151-158, 2007 (antibodies against VHSV).pdf
DAO 74, 151-158, 2007 (antibodies against VHSV).pdf
DAO 74, 151-158, 2007 (antibodies against VHSV)
application/pdf
74217
https://buleria.unileon.es/bitstream/10612/9024/1/DAO%2074%2c%20151-158%2c%202007%20%28antibodies%20against%20VHSV%29.pdf
5fd4926286b290e85a6587bb3b9898c6
MD5
1
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1413
https://buleria.unileon.es/bitstream/10612/9024/2/license.txt
2afe00e6e6eabe127e18eca38b9388ab
MD5
2
10612/9024
oai:buleria.unileon.es:10612/9024
2020-12-10 10:00:28.15
BULERIA. Repositorio Institucional de la Universidad de León
buleria@unileon.es
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