RT info:eu-repo/semantics/article
T1 Cryopreservation of Iberian red deer (Cervus elaphus hispanicus) spermatozoa obtained by electroejaculation
A1 Martínez Pastor, Felipe
A1 Martínez, Félix
A1 Álvarez García, Mercedes
A1 Maroto Morales, Alejandro
A1 García Álvarez, Olga
A1 Soler, Ana J.
A1 Garde López-Brea, Julián
A1 Paz Cabello, Paulino de
A1 Anel Rodríguez, Luis
A2 Biologia Celular
K1 Veterinaria
K1 Sperm cryopreservation
K1 Electroejaculation
K1 Extenders
K1 Red deer
K1 Semen
AB We tested extenders and freezing protocols for Iberian red deer semen. Samples were obtained by electroejaculation (10 stags), and analyzed for motility (CASA), viability (propidium ioide), acrosomal (PNA-FITC) and mitochondrial status (JC-1). Samples were diluted in extender, cooled and adjusted for glycerol (extender with higher glycerol concentration), brought to  mL−1 and frozen. Four experiments were carried out, repeating sperm analysis after thawing to compare treatments. In a first experiment, seven samples were frozen using Triladyl® (20% egg yolk) and UL extender (Tes–Tris–fructose, 15% egg yolk, 4% glycerol). Triladyl® yielded higher motility after thawing. In a second trial, 17 samples were frozen using Triladyl®, Andromed®, Bioxcell®, and UL with 8% LDL (low-density lipoproteins). Triladyl® and Andromed® performed better than Bioxcell® on motility, and than UL-LDL on viability and acrosomal status. In a third experiment, the performance of freezing the sperm-rich ejaculate fraction versus the whole ejaculate was tested on nine samples. The sperm-rich ejaculate fraction not only rendered more motile and viable spermatozoa but also showed higher freezability (higher motile spermatozoa recovery). In a fourth experiment, we tried three modifications of the freezing protocol, for improving the freezability of low concentration samples: prior removal of seminal plasma; replacing extender (second fraction) for pure glycerol to reduce dilution; and performing only the dilution, not the second dilution. No differences were found, although only three samples could be used. Both Triladyl® and Andromed® were deemed appropriate for freezing Iberian red deer semen, and the rich fraction should be selected for freezing.
PB Elsevier
YR 2019
FD 2019-04-17
LK http://hdl.handle.net/10612/10288
UL http://hdl.handle.net/10612/10288
NO Theriogenology, 2009, vol. 71, n. 4
NO P. 628-638
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 26-abr-2024