RT info:eu-repo/semantics/article
T1 DNA Status on Thawed Semen from Fighting Bull: A Comparison Between the SCD and the SCSA Tests
A1 Martínez Pastor, Felipe
A1 Fernández Santos, María Rocío
A1 Domínguez Rebolledo, Álvaro Efrén
A1 Esteso, Milagros
A1 Garde López-Brea, Julián
A2 Biologia Celular
K1 Veterinaria
K1 Toros de lidia
K1 Semen
K1 Cromatina
K1 Estrés oxidativo
AB The assessment of sperm chromatin status is compulsory in a complete spermiogram. Here we applied the sperm chromatin structure assay (SCSA) and the sperm chromatin dispersion (SCD) test to assess the chromatin status of three fighting bulls. Cryopreserved semen (two straws/bull) were analysed by duplicate after thawing and after 6 h at 37°C with and without oxidative stress (1 mm FE2+). Results (SCD: percentage of spermatozoa with halo; SCSA: SD‐DFI, %DFI and HDS) were analysed for differences between bulls and treatments, sensitivity and specificity (receiver operating characteristic curves) and repeatability (repeatability coefficients as 2SD of duplicate differences).%DFI for the three bulls was below 2% at 0 h, indicating no risk for fertility according to previous reports. It increased slightly for two of the bulls after FE2+ treatment (%DFI < 5%) and more pronouncedly for the other bull (C, %DFI∼10%), which merits further investigation. SCD rendered higher percentage of halos for bull C, but could not discriminate between samples with and without oxidizing treatment (AUC: 0.52). SCSA (%DFI) showed a high discriminating ability between treatments (AUC: 0.96). The repeatability coefficient was also higher for SCD (5.9) than for %DFI (1.8), indicating lower repeatability for SCD. Overall, %DFI might be the most useful parameter for assessing sperm chromatin on fighting bull. SCD might yield different information than SCSA, hence further research is warranted.
PB John Wiley & Sons
YR 2019
FD 2019-04-17
LK http://hdl.handle.net/10612/10291
UL http://hdl.handle.net/10612/10291
NO Reproduction in Domestic Animals, 2009, vol. 44, n. 3
NO P. 424-431
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 27-abr-2024