RT info:eu-repo/semantics/article
T1 Probes and Techniques for Sperm Evaluation by Flow Cytometry
A1 Martínez Pastor, Felipe
A1 Mata Campuzano, María
A1 Álvarez Rodríguez, Manuel
A1 Álvarez García, Mercedes
A1 Anel Rodríguez, Luis
A1 Paz Cabello, Paulino de
A2 Biologia Celular
K1 Veterinaria
K1 Citometría de flujo
K1 Esperma
AB Flow cytometry has become an important technique in sperm evaluation and is increasingly used both for routine assessment and for research in veterinary science. We have revised the literature, describing fluorescent probes that have been used for analysing spermatozoa by flow cytometry, regarding: viability, acrosomal status, capacitation, mitochondrial status, apoptotic markers, oxidative stress markers, DNA damage, sperm counting and sperm sizing. Details and problems of some techniques are reviewed, with special attention to the occurrence of non‐sperm particles in the samples (‘debris’). New and promising aspects of flow cytometry, such as sperm sorting using viability markers as selection criteria, are highlighted. The relationship between flow cytometry analyses and fertility and their future improvements are considered.
PB John Wiley & Sons
YR 2019
FD 2019-04-17
LK http://hdl.handle.net/10612/10293
UL http://hdl.handle.net/10612/10293
NO Reproduction in Domestic Animals, 2010, vol. 45, s. 2
NO P. 67-78
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 25-abr-2024