RT info:eu-repo/semantics/article
T1 Detection of ‘long-haired’ Saprolegnia (S. parasitica) isolates using monoclonal antibodies
A1 Fregeneda Grandes, Juan Miguel
A1 Rodríguez Cadenas, Fausto
A1 Carbajal González, María Teresa
A1 Aller Gancedo, José Miguel
A2 Sanidad Animal
K1 Sanidad animal
K1 Veterinaria
K1 Truchas
K1 Saprolegnia parasítica
K1 Inmunoglobulinas
AB The ability of five monoclonal antibodies (Mabs) raised against a pathogenic Saprolegniaparasitica isolate from brown trout to detect and differentiate between isolates withbundles of long hairs (S. parasitica) and other Saprolegnia species was determined by meansof an indirect immunofluorescence assay. Four of the Mabs used recognized some of thelong-haired S. parasitica isolates but also cross-reacted with other Saprolegnia species without bundles of hairs and with Achlya sp. The other Mab (named 18A6) was able to differentiate between the asexual and most of the sexual isolates in the group of long-hairedS. parasitica isolates, but did not recognize Achlya sp. or the Saprolegnia species withoutbundles of hairs, with the exception of S. hypogyna. These results indicate that isolateswith bundles of long hairs are closely related with other members of genus Saprolegniaand share several antigens. However, Mab 18A6 seems to recognize an epitope that isexpressed mainly in the asexual isolates in the long-haired S. parasitica isolates.
PB Elsevier
YR 2019
FD 2019-04-17
LK http://hdl.handle.net/10612/10294
UL http://hdl.handle.net/10612/10294
NO Mycological research, 2007, vol. 111, n.  6
NO P. 726-733
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 26-abr-2024