RT info:eu-repo/semantics/article
T1 Post mortem time and season alter subpopulation characteristics of Iberian red deer epididymal sperm
A1 Martínez Pastor, Felipe
A1 Díaz Corujo, Ana Rocío
A1 Anel Rodríguez, Enrique
A1 Herráez Ortega, María Paz
A1 Anel Rodríguez, Luis
A1 Paz Cabello, Paulino de
A2 Biologia Celular
K1 Veterinaria
K1 Post mortem
K1 Seasonality
K1 Epididymis
K1 Sperm subpopulations
K1 Red deer
AB We have studied the effect of post mortem time and season on sperm subpopulation pattern and characteristics. We used epididymal samples from free-ranging Iberian red deers harvested during the hunting season. We studied samples at different moments of the year (rut, transition period and post-rut), and at different times post mortem (up to 4 days). Sperm were extracted from the cauda epididymis and their motility was evaluated by means of a CASA system. A principal component and clustering analysis were carried out to identify subpopulations. Post mortem time caused a significant decrease in motility quality, and a general deterioration in subpopulation characteristics. We found three subpopulations the first day, and the one indicating good sperm quality decreased with post mortem time until it disappeared on the fourth day. This may indicate considerable impairment of the samples after 72 h post mortem, which could compromise their use in AI programs. With regard to season, subpopulation pattern and characteristics were better in the transition and post-rut periods. Moreover, we found one subpopulation formed by mature spermatozoa, which increased from rut to post-rut. This might be a negative fact, because samples collected after the rut may undergo hypermaturation, which possibly impairs fertility. Our results are of interest for the management of wildlife germplasm banks based on post mortem sperm recovery.
PB Elsevier
YR 2019
FD 2019-04-17
LK http://hdl.handle.net/10612/10314
UL http://hdl.handle.net/10612/10314
NO Theriogenology, 2005, vol. 64, n. 4
NO P. 958-974
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 14-may-2024