RT info:eu-repo/semantics/article
T1 Refrigerated Storage of Red Deer Epididymal Spermatozoa in the Epididymis, Diluted and with Vitamin C Supplementation
A1 Fernández Santos, María Rocío
A1 Domínguez Rebolledo, Álvaro Efrén
A1 Esteso, Milagros
A1 Garde López-Brea, Julián
A1 Martínez Pastor, Felipe
A2 Biologia Celular
K1 Veterinaria
K1 Esperma
K1 Refrigeración
K1 Ciervo rojo
K1 Cromatina
K1 CASA
K1 Epidídimo
K1 Vitamina C
AB We have approached the problem of refrigerated storage of epididymal sperm samples from red deer by comparing three options: storing the genital (testicles within the scrotum), diluting the semen in extender or diluting the semen in extender supplemented with an anti‐oxidant. Twenty‐nine pairs of testes were collected. Spermatozoa from one of each of the pairs were immediately recovered, and diluted to 400 × 106 sperm/ml in Tris‐citrate‐fructose with 20% egg yolk. Control group was stored as such, and Anti‐oxidant group was supplemented with 0.8 mm vitamin C. The remaining epididymides and the diluted samples were stored at 5°C and spermatozoa were analysed at 0, 24, 96 and 192 h for: motility [computer‐assisted semen analysis (CASA)], acrosomal integrity, sperm viability (eosine/nigrosine staining), normal tails and chromatin status [sperm chromatin structure assay (SCSA)]. In general, seminal quality decreased with storage time. Vitamin C supported progressive motility better at 24 h (median 42% vs 23% Control and 15% epididymis), reduced the incidence of tail abnormalities and protected chromatin. Storing the semen in the epididymis slowed down motility loss, but slightly increased the occurrence of tail abnormalities and viability was lower at 192 h. However, regarding chromatin status, sperm stored in the epididymis was protected similarly to those diluted in the medium supplemented with vitamin C. Although the differences between the three groups were small, there were some advantages in supplementing the extender with vitamin C. Besides, refrigerating the epididymis may be a good option when immediate processing is not available.
PB John Wiley & Sons
YR 2019
FD 2019-04-17
LK http://hdl.handle.net/10612/10321
UL http://hdl.handle.net/10612/10321
NO Reproduction in Domestic Animals, 2009, vol. 44, n. 2
NO P. 212-220
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 27-abr-2024