RT info:eu-repo/semantics/article
T1 Effect of storage method and extender osmolality in the quality of cryopreserved epididymal ram spermatozoa
A1 Tamayo Canul, Julio Renan
A1 Álvarez García, Mercedes
A1 Mata Campuzano, María
A1 Álvarez Rodríguez, Manuel
A1 Paz Cabello, Paulino de
A1 Anel Rodríguez, Luis
A1 Martínez Pastor, Felipe
A2 Biologia Celular
K1 Veterinaria
K1 Ram
K1 Extender
K1 Osmolality
K1 Sperm quality
K1 Cryopreservation
AB Post-mortem sperm recovery and cryopreservation could be a complement to germplasm banking in sheep, especially for endangered breeds. This study is an attempt to identify factors for improving the success of cryopreserving ram epididymal spermatozoa, considering the decrease of sperm quality with post-mortem time. Epididymal spermatozoa from 9 rams were kept at 5 °C using three storage methods: within the epididymes, undiluted sperm mass, and diluted in extenders of different osmolality (TES–Tris–fructose at 320, 370 or 420 mOsm/kg, 20% egg yolk, 8% glycerol). At 0, 24, 48 and 72 h, spermatozoa were cryopreserved using each extender. Samples were analyzed before and after cryopreservation by CASA (motility) and flow cytometry (viability and acrosomal status). Post-mortem time decreased pre-freezing and post-thawing sperm quality. Some storage × extender combinations improved the effect of post-mortem time on sperm quality. Both epididymis storage combined with the 420 extender, and storing the spermatozoa diluted in the 320 extender improved post-thawing quality, especially at long post-mortem times. Storing the spermatozoa diluted in the 370 extender was detrimental for the acrosomal status. These findings have practical applications. The simplest storage method (within the epididymes) seems to be adequate if hyperosmotic extenders were used for freezing. An alternative method could be storing the spermatozoa diluted in a hypoosmotic extender. These recommendations are limited to the osmolalities tested in this study (420 mOsm/kg and 320 mOsm/kg); other osmolalities should be tested.
PB Elsevier
YR 2019
FD 2019-04-17
LK http://hdl.handle.net/10612/10334
UL http://hdl.handle.net/10612/10334
NO Animal Reproduction Science, 2011, vol. 129, n. 3–4
NO P. 188-199
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 25-abr-2024