RT info:eu-repo/semantics/article
T1 A pilot study on post-thawing quality of Iberian red deer spermatozoa (epididymal and electroejaculated) depending on glycerol concentration and extender osmolality
A1 Martínez Pastor, Felipe
A1 Martínez, Félix
A1 García Macías, Vanesa
A1 Esteso, Milagros
A1 Anel Rodríguez, Enrique
A1 Fernández Santos, María Rocío
A1 Soler, Ana J.
A1 Paz Cabello, Paulino de
A1 Garde López-Brea, Julián
A1 Anel Rodríguez, Luis
A2 Biologia Celular
K1 Veterinaria
K1 Sperm cryopreservation
K1 Epididymis
K1 Electroejaculation
K1 Extender
K1 Red deer
AB The optimization of cryopreservation extenders is a fundamental issue for adequately performing germplasm banking on wild species. We have tested two glycerol concentrations (4 and 8%), and three extender osmolalities (320, 380 and 430 mOsm/kg; before adding cryoprotectants), for cryopreservation of epididymal and ejaculated sperm samples from Iberian red deer. All the extenders were based on Tes–Tris and fructose (for osmolality adjustment), and complemented with 20% egg yolk. Epididymal and ejaculated sperm samples were obtained from the cauda epididymis (post-mortem) and using electroejaculation, respectively. Samples were diluted 1:1 with each extender and equilibrated for 2 h at 5 °C. Then, they were diluted down to 100 × 106 sperm/mL and frozen at −20 °C/min. Post-thawed samples were assessed for motility (CASA), HOS test, proportion of swollen (osmotically challenged) cells in the untreated sample, viability and acrosomal status. For epididymal samples, 8% glycerol rendered a slightly higher proportion of intact acrosomes on viable spermatozoa than 4%; regarding extender osmolality, 380 and 430 mOsm/kg rendered higher motility results, and the 430 mOsm/kg yielded the lowest proportion of swollen spermatozoa. For ejaculated samples, 4% glycerol yielded more viable spermatozoa than 8%; for extender osmolality, 320 mOsm/kg rendered the highest percentages of progressively motile and viable spermatozoa, although 380 mOsm/kg extender was not significantly different. These results show that sample source influences extender suitability, and that extenders should be isoosmotic or rather slightly hyperosmotic. Future studies should test multiple glycerol concentrations and extender osmolalities in order to adjust them to these kinds of sample.
PB Elsevier
YR 2019
FD 2019-04-17
LK http://hdl.handle.net/10612/10337
UL http://hdl.handle.net/10612/10337
NO Theriogenology, 2006, vol. 66, n. 5
NO P. 1165-1172
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 19-abr-2024