RT info:eu-repo/semantics/article
T1 Clinical evaluation and antibody responses in sheep after primary and secondary experimental challenges with the mange mite Sarcoptes scabiei var. ovis
A1 Rodríguez Cadenas, Fausto
A1 Carbajal González, María Teresa
A1 Fregeneda Grandes, Juan Miguel
A1 Aller Gancedo, José Miguel
A1 Rojo Vázquez, Francisco Antonio
A2 Sanidad Animal
K1 Sanidad animal
K1 Veterinaria
K1 Inmunoglobulinas
K1 Sarna sarcóptica
K1 Ganado ovino
AB In this work the clinical evolution and the specific serum IgG and IgE antibody responses insheep after primary (n = 10) and secondary (n = 4) experimental challenges with themange mite Sarcoptes scabiei var. ovis were studied. The primary infection wascharacterized by the development of mange lesions in all sheep, a detection of live S.scabiei mites in 70% skin scrapings taken in week 10 post-challenge (PC), strongly raisedand sustained specific IgG levels and a more moderate but continuous rise in specific IgElevels. Seroconversion was detected for IgG and IgE by ELISA in 90% and 60% of the sheep inweek 8 PC, respectively. By Western-blotting (WB), ten IgG-reactive bands (36–120 kDa)and four IgE-reactive bands (90–180 kDa) were observed in week 8 PC. Following thesecondary challenge the ewes developed a smaller area of mange lesion than that seenfollowing primary challenge and live S. scabiei mites were not detected in skin scrapingscollected in week 8 PC, suggesting that sheep had developed immunity to re-infection.Compared to primary infection, the specific IgG secondary antibody levels were transient,but in contrast there was an anamnestic IgE response, resulting in an elicitation of specificserum IgE levels in week 2 PC significantly higher than those demonstrated after primaryinfection. WB analysis revealed one additional IgG-reactive band (180 kDa) and noadditional IgE-reactive bands. Determining the immunodiagnostic or vaccination value ofthe IgG-reactive antigens and IgE-reactive allergens detected requires further studies.
PB Elsevier
YR 2019
FD 2019-04-17
LK http://hdl.handle.net/10612/10369
UL http://hdl.handle.net/10612/10369
NO Veterinary Immunology and Immunopathology, 2010, vol. 133, n. 2-4
NO P. 109-116
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 25-abr-2024