RT info:eu-repo/semantics/article
T1 Undiluted or extended storage of ram epididymal spermatozoa as alternatives to refrigerating the whole epididymes
A1 Tamayo Canul, Julio Renan
A1 Álvarez García, Mercedes
A1 López Urueña, Elena
A1 Nicolás, M.
A1 Martínez Pastor, Felipe
A1 Anel Rodríguez, Enrique
A1 Anel Rodríguez, Luis
A1 Paz Cabello, Paulino de
A2 Biologia Celular
K1 Veterinaria
K1 Ram
K1 Epididymis
K1 Refrigerated storage
K1 Osmolality
K1 Extender
K1 Sperm quality
AB The effect of storage procedure at 5 °C on the quality of ram spermatozoa from the cauda epididymis was analyzed. Two strategies were tested at 0, 24, 48 and 72 h post-mortem: (1) spermatozoa held in the epididymal fluid and stored either in the cauda epididymis (In-EPID) or in vitro (Ex-EPID), (2) epididymal spermatozoa extended in three media at 320, 370 and 420 mOsm/kg (D320, D370, D420). Analyzed parameters were: osmolality, pH, motility, acrosomal status and viability. In experiment 1, osmolality of the In-EPID samples, but not in Ex-EPID, increased with post-mortem time. Motility of In-EPID spermatozoa in samples, after 24 h post-mortem, was higher compared to the Ex-EPID samples, although differences decreased at 48 and 72 h. In experiment 2, total (TM) and progressive motility (PM) were not significantly affected by storage time for D320 and In-EPID samples. However, the motility of D370 and D420 samples significantly decreased with time. TM and PM of D320 were significantly higher than D370 and D420 at 72 h. At 24 h, sperm viability was higher for In-EPID (80.7 ± 3.4%) than for the extended samples (44.8 ± 2.9%, 37.7 ± 3.9% and 48.6 ± 6.0% for D320, D370 and D420, respectively), which also decreased faster with time. At 24 h, the percentage of damaged acrosomes was low and similar for the four methods of storage, but damaged acrosomes increased with time for D320 and D370. Storing the spermatozoa in the epididymis is a good strategy for maintaining sperm quality in ram, at least for 48 h. The D320 extender preserve motility of epididymal spermatozoa but does not protect the status of the acrosome.
PB Elsevier
YR 2019
FD 2019-04-22
LK http://hdl.handle.net/10612/10533
UL http://hdl.handle.net/10612/10533
NO Animal Reproduction Science, 2011 vol. 126, n. 1–2
NO P. 76-82
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 19-abr-2024