RT info:eu-repo/semantics/article
T1 Sperm parameters on Iberian red deer: Electroejaculation and post-mortem collection
A1 Martínez, A. F.
A1 Martínez Pastor, Felipe
A1 Álvarez García, Mercedes
A1 Fernández Santos, María Rocío
A1 Esteso, Milagros
A1 Paz Cabello, Paulino de
A1 Garde López-Brea, Julián
A1 Anel Rodríguez, Luis
A2 Biologia Celular
K1 Veterinaria
K1 Red deer
K1 Epididymal spermatozoa
K1 Electroejaculation
K1 Sperm evaluation
K1 Testicular growth
AB Artificial reproductive technologies (ART) for cervids have improved, but a need remains for the collection of basic data. We studied two models of sperm collection in Iberian red deer, post-mortem (PM) in a wild population (179 samples) and by electroejaculation (EE) in a farmed population (37 samples), recording: testicular and epididymal weight, testicular diameter, sperm quantity, pH and osmolality and spermatozoa quality (motility by CASA, abnormal forms, cytoplasmic droplets, viability and acrosomal status). We tested the relationship of these parameters with stag age and compared the two models (PM and EE; medians showed). Genitalia parameters were linearly related to stag age (testicular diameter: 31.5–50.5 mm for 2–9 years). Total number of spermatozoa collected were PM: 2.5 × 109 and EE: 3.6 × 109 (P > 0.05), increasing with age only for PM. We found a positive relationship between testicular size and spermatozoa collected for PM. Osmolality and pH were PM: 6.28 and 378 mOsm/kg; EE: 7.63 and 309 mOsm/kg (P < 0.05). The pH increased with age only for EE. Percentage of motile spermatozoa was similar for PM and EE, but motility quality was lower for PM. Abnormal forms, proximal and distal droplets were lower for EE (22%, 1.3%, 1.5% vs. PM: 23%, 4.3%, 83%). Viability was similar (74%) and intact acrosomes were higher for EE (97% vs. 89%). Both PM and EE samples could be used for germplasm banking. This study contributes with new data on red deer spermatology and for the development of ART in cervids.
PB Elsevier
YR 2019
FD 2019-04-23
LK http://hdl.handle.net/10612/10609
UL http://hdl.handle.net/10612/10609
NO Theriogenology, 2008, vol. 70, n. 2
NO P. 216-226
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 26-abr-2024