RT info:eu-repo/semantics/article
T1 Reduced glutathione and Trolox (vitamin E) as extender supplements in cryopreservation of red deer epididymal spermatozoa
A1 Anel López, Luis
A1 Álvarez Rodríguez, Manuel
A1 García Álvarez, Olga
A1 Álvarez García, Mercedes
A1 Maroto Morales, Alejandro
A1 Anel Rodríguez, Luis
A1 Paz Cabello, Paulino de
A1 Garde López-Brea, Julián
A1 Martínez Pastor, Felipe
A2 Biologia Celular
K1 Veterinaria
K1 Epididymal sperm
K1 Red deer
K1 Antioxidant
K1 Trolox
K1 GSH
K1 Cryopreservation
AB The use of assisted reproductive techniques in cervids is increasing as the commercial use of these species increase. We have tested the suitability of the antioxidants Trolox and reduced glutathione (GSH) for freezing red deer epididymal spermatozoa, aiming at improving post-thawing quality. Samples from 19 stags were frozen in a TES-Tris-fructose extender (20% egg yolk, 8% glycerol), with 1 or 5 mM of antioxidant. Motility (CASA), lipoperoxidation (malondialdehyde –MDA– production), membrane status, mitochondrial activity, acrosomal status (flow cytometry) and chromatin status (SCSA: %DFI and %HDS; flow cytometry) were assessed after thawing and after 6 h at 39 °C. There were few differences between treatments after thawing, with Trolox reducing MDA production in a dose–response manner. After the incubation, sperm quality decreased and %DFI increased moderately, with no change for MDA. GSH improved motility, kinematic parameters and mitochondrial status, with a slight increase in %HDS. GSH 5 mM also increased moderately MDA production and %DFI, possibly due to enhanced metabolic activity and reducing power. Trolox maintained MDA low, but was detrimental to sperm quality. Trolox might not be appropriate for the cryopreservation of red deer epididymal spermatozoa, at least at the millimolar range. GSH results are promising, especially regarding motility improvement after the post-thawing incubation, and should be selected for future fertility trials.
PB Elsevier
YR 2019
FD 2019-04-25
LK http://hdl.handle.net/10612/10658
UL http://hdl.handle.net/10612/10658
NO Animal Reproduction Science, 2012, vol. 135, n. 1–4
NO P. 37-46
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 25-abr-2024