RT info:eu-repo/semantics/article
T1 Optimization of Glycerol Concentration and Freezing Rate in the Cryopreservation of Ejaculate From Brown Bear (Ursus arctos)
A1 Paz Cabello, Paulino de
A1 Álvarez Rodríguez, Manuel
A1 Nicolás, M.
A1 Álvarez García, Mercedes
A1 Chamorro Álvarez, César Ángel
A1 Barragán Santos, Santiago
A1 Martínez Pastor, Felipe
A1 Anel Rodríguez, Luis
A2 Biologia Celular
K1 Veterinaria
K1 Glicerina
K1 Criopreservación
K1 Esperma
K1 Osos pardos
K1 Ursus arctos
AB In order to establish a semen bank for the endangered Cantabrian brown bear, we tested five glycerol concentrations and three freezing rates for electroejaculated semen. Electroejaculation was performed on nine males. Semen was diluted in TES–Tris–Fructose (20% egg yolk, 2% EDTA, 1% Equex) with 2%, 4%, 6%, 8% or 10% glycerol and frozen at −10, −20 or −40°C/min. Before and after cryopreservation, samples were analysed for motility (CASA), viability and acrosomal status (flow cytometry). Pre‐freezing results showed that glycerol concentration had no significant effect on total motility or progressive motility, but it significantly decreased VCL, ALH, viability and acrosomal status (p < 0.05). After thawing, sperm motility was higher at extender with 4%, 6% and 8% glycerol, but only at 4% and 6% glycerol for viability and acrosomal status. For 4% and 6% glycerol, freezing rates did not have significant effects. The curve fitting gave an estimate of the optimal glycerol concentration, with all the optimal values for every parameter between 6% and 7% glycerol falling. We propose using 6% glycerol and a freezing velocity of −20°C/min for freezing brown bear ejaculated spermatozoa.
PB John Wiley & Sons
YR 2019
FD 2019-04-25
LK http://hdl.handle.net/10612/10663
UL http://hdl.handle.net/10612/10663
NO Reproduction in Domestic Animals, 2012, vol. 47, n. 1
NO P. 105-112
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 24-abr-2024