RT info:eu-repo/semantics/article
T1 Effect of length of time post-mortem on quality and freezing capacity of Cantabric chamois (Rupicapra pyrenaica parva) epididymal spermatozoa
A1 Álvarez Rodríguez, Manuel
A1 Álvarez García, Mercedes
A1 Anel López, Luis
A1 Guerra, Camino
A1 Chamorro Álvarez, César Ángel
A1 Anel Rodríguez, Luis
A1 Paz Cabello, Paulino de
A1 Martínez Pastor, Felipe
A2 Biologia Celular
K1 Veterinaria
K1 Cantabric chamois
K1 Cryopreservation
K1 Epididymal sperm
K1 Post-mortem time
K1 Germplasm banking
AB Genome Resource Banks are keystones in the ex-situ conservation of wild species. Post-mortem (PM) collection of epididymal spermatozoa is an opportunistic and valuable source of germplasm, the time from the death of the animal limits its use. Seeking to improve germplasm preservation strategies for the chamois (Rupicapra sp.), the effect of PM time on epididymal sperm quality and freezability was studied using the Cantabrian chamois. Samples were classified according to PM collection time, up to 216 h (refrigerated), and cryopreserved (Tris-citric acid-fructose, 430 mOsm/kg, 15% egg yolk, 8% glycerol; freezing at −20 °C/min). Sperm quality was assessed after recovery and post-thawing (motility by CASA, HOS test, abnormal forms, cytoplasmic droplets, and viability and acrosomal damage by flow cytometry). The sperm mass pH and osmolality showed a positive correlation with time. Total sperm motility dropped after 2 days PM, with progressivity and sperm velocities remained similar up to 3 days PM. Sperm freezability was acceptable, with the post-thawing HOST, motility, progressivity, VAP, VCL, VSL and BCF negatively correlating with PM time. Overall, chamois epidydimal samples were not adequate for preservation after 6 days PM. Freezability capacity could make these spermatozoa suitable for specific ART even if kept refrigerated for several days PM.
PB Elsevier
YR 2019
FD 2019-05-03
LK http://hdl.handle.net/10612/10666
UL http://hdl.handle.net/10612/10666
NO Animal Reproduction Science, 2018,  vol. 198
NO P. 184-192
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 19-abr-2024