RT info:eu-repo/semantics/article
T1 Quality, oxidative markers and DNA damage (DNA) fragmentation of red deer thawed spermatozoa after incubation at 37 °C in presence of several antioxidants
A1 Mata Campuzano, María
A1 Álvarez Rodríguez, Manuel
A1 Olmo de Medina, Enrique del
A1 Fernández Santos, María Rocío
A1 Garde López-Brea, Julián
A1 Martínez Pastor, Felipe
A2 Biologia Celular
K1 Veterinaria
K1 Red deer
K1 Spermatozoa
K1 Antioxidant
K1 Oxidative stress
K1 DNA damage
AB Antioxidants may be useful for supplementing sperm extenders. We have tested dehydroascorbic acid (DHA), TEMPOL, N-acetyl-cysteine (NAC) and rutin on epididymal spermatozoa from red deer, during incubation at 37 °C. Cryopreserved spermatozoa were thawed, washed and incubated with 1 mm or 0.1 mm of each antioxidant, including oxidative stress (Fe2+/ascorbate). Motility (CASA and clustering of subpopulations), viability, mitochondrial membrane potential, and acrosomal status were assessed at 2 and 4 h. Lipoperoxidation, intracellular reactive oxygen species (ROS) and DNA damage (DNA) status (TUNEL) were checked at 4 h. Oxidative stress increased ROS, lipoperoxidation and DNA damage. Overall, antioxidants negatively affected motility and physiological parameters. Only DHA 1 mm protected motility, increasing the fast and progressive subpopulation. However, it had a detrimental effect on acrosomal and DNA status, in absence of oxidative stress. Tempol and rutin efficiently reduced lipoperoxidation, ROS, and DNA damage in presence of oxidative stress. NAC was not as efficient as TEMPOL or rutin reducing lipoperoxidation or protecting DNA, and did not reduce ROS, but its negative effects were lower than the other antioxidants when used at 1 mm, increasing the subpopulation of hyperactivated-like spermatozoa at 2 h. Our results show that these antioxidants have mixed effects when spermatozoa are incubated at physiological temperatures. DHA may not be suitable because of prooxidant effects, but TEMPOL, NAC and rutin may be considered for cryopreservation trials. In general, exposure of red deer spermatozoa to these antioxidants should be limited to low temperatures, when only protective effects may develop.
PB Elsevier
YR 2019
FD 2019-05-07
LK http://hdl.handle.net/10612/10670
UL http://hdl.handle.net/10612/10670
NO Theriogenology, 2012 vol. 78, n. 5
NO P. 1005-1019
DS BULERIA. Repositorio Institucional de la Universidad de León
RD Jul 11, 2024