RT info:eu-repo/semantics/article
T1 Taking advantage of the use of supervised learning methods for characterization of sperm population structure related with freezability in the Iberian red deer
A1 Ramón Fernández, Manuel
A1 Martínez Pastor, Felipe
A1 García Álvarez, Olga
A1 Maroto Morales, Alejandro
A1 Soler, Ana J.
A1 Jiménez Rabadán, Pilar
A1 Fernández Santos, María Rocío
A1 Bernabéu Cañete, Rodolfo
A1 Garde López-Brea, Julián
A2 Biologia Celular
K1 Veterinaria
K1 Sperm subpopulations
K1 Support vector machines
K1 Sperm freezability
K1 Iberian red deer
AB Using Iberian red deer as a model, this study presents a supervised learning method, the Support Vector Machines (SVM), to characterize sperm population structure related with freezability. Male freezability was assessed by evaluating motility, membrane status and mitochondrial membrane potential of sperm after a freezing-thawing procedure. The SVM model was generated using sperm motility information captured by computer-assisted sperm analysis (CASA) from thawed semen, belonging to six stags with marked differences on their freezability. A total of 1369 sperm tracks were recorded for seven kinematic parameters and assigned to four motility patterns based on them: weak motile, progressive, transitional and hyperactivated-like. Then, these data were split in two sets: the training set, used to train the SVM model, and the testing set, used to examine how the SVM method and three other unsupervised methods, a non-hierarchical, a hierarchical and a multistep clustering procedures, performed the sperm classification into subpopulations. The SVM was revealed as the most accurate method in the characterization of sperm subpopulations, showing all the sperm subpopulations obtained in this way high significant correlations with those sperm parameters used to characterize freezability of males. Given its superiority, the SVM method was used to characterize the sperm motile subpopulations in Iberian red deer. Sperm motile data from frozen–thawed semen belonging to 25 stags were recorded and loaded into the SVM model. The sperm population structure revealed that those males showing poor freezability were characterized by high percentages of sperm with a weak motility pattern. In opposite, males showing good freezability were characterized by higher percentages of sperm with a progressive and hyperactivated-like motility pattern and lower percentages of sperm with a weak motile pattern. We also identified a sperm subpopulation with a transitional motility pattern. This subpopulation increased as the freezability of males improved, and may be used as indicative of overall sperm motility.
PB Elsevier
YR 2019
FD 2019-05-07
LK http://hdl.handle.net/10612/10672
UL http://hdl.handle.net/10612/10672
NO Theriogenology, 2012, vol. 77, n. 8
NO P. 1661-1672
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 25-abr-2024