RT info:eu-repo/semantics/article T1 Fertility of cryopreserved ovine semen is determined by sperm velocity A1 Olmo de Medina, Enrique del A1 Bisbal Vigo, Alfonso A1 Maroto Morales, Alejandro A1 García Álvarez, Olga A1 Ramón Fernández, Manuel A1 Jiménez Rabadán, Pilar A1 Martínez Pastor, Felipe A1 Soler, Ana J. A1 Garde López-Brea, Julián A1 Fernández Santos, María Rocío A2 Biologia Celular K1 Veterinaria K1 Fertility K1 Cryopreserved semen K1 Ram K1 Flow cytometry K1 CASA AB The present study aims to examine the predictive value of some sperm parameters on male fertility. Semen samples from six Manchega rams were collected and cryopreserved. Sperm quality was assessed after thawing and after 2 h of incubation, either in the freezing extender (37 °C) or after dilution in Synthetic Oviductal Fluid (SOF) (38 °C, 5% CO2), attempting to mimic the physiological conditions of the female reproductive tract. The following sperm parameters were evaluated: motility and kinetic parameters by computer-assisted semen analyzer (CASA), and sperm viability (propidium iodide), mitochondrial membrane potential (JC-1), apoptotic-like membrane changes (YO-PRO-1), acrosomal status (PNA-FITC), and intracellular calcium (fluo-3) by flow cytometry. Results showed no significant differences between incubation media neither after thawing nor after incubation. There were no significant correlations between fertility and sperm parameters assessed by flow cytometry. However, after incubation in the freezing extender, sperm samples from males with poor fertility yielded less linearity and velocity (P < 0.05) as indicated by motility parameters analyzed by CASA. These results indicate that kinematic sperm motility parameters evaluation by CASA might be useful to identify samples with poor fertility. PB Elsevier YR 2019 FD 2019-05-07 LK http://hdl.handle.net/10612/10677 UL http://hdl.handle.net/10612/10677 NO Animal Reproduction Science, 2013, vol. 138, n. 1–2 NO P. 102-109 DS BULERIA. Repositorio Institucional de la Universidad de León RD 26-abr-2024