RT info:eu-repo/semantics/article
T1 Extender osmolality, glycerol and egg yolk on the cryopreservation of epididymal spermatozoa for gamete banking of the Cantabric Chamois (Rupicapra pyrenaica parva)
A1 Martínez Pastor, Felipe
A1 Álvarez García, Mercedes
A1 Guerra, Camino
A1 Chamorro Álvarez, César Ángel
A1 Anel López, Luis
A1 Paz Cabello, Paulino de
A1 Anel Rodríguez, Luis
A1 Álvarez Rodríguez, Manuel
A2 Biologia Celular
K1 Veterinaria
K1 Cantabrian chamois
K1 Cryopreservation
K1 Epididymal spermatozoa
K1 Extender
K1 Osmolality
AB Germplasm banking is a key technology enabling the ex-situ conservation of wild species. However, cryopreservation protocols must be tested to assure the applicability of the banked material. The objective of this study was defining a range of parameters for the composition of a semen extender for Cantabrian chamois epididymal spermatozoa (post-mortem collection). The freezing extender was based in a TES-Tris-fructose buffer, modifying its composition in three experiments: Osmolality of the buffer (320, 380 or 430 mOsm/kg, 8% glycerol, 15% egg yolk), glycerol (4 or 8%, 430 mOsm/kg, 15% egg yolk), egg yolk (10 or 15%, 430 mOsm/kg, 4% glycerol). Sperm was extended at 100 mill. spermatozoa/ml, cooled at 5 °C and frozen at −20 °C/min. Sperm quality was assessed pre and post-thawing (CASA, HOS test, abnormal forms, cytoplasmic droplets, and viability and acrosomal damage by flow cytometry). Freezability was good overall, with total motility of 65.5% ± 2.4 initial and 55.8% ± 2.4 post-thawing. The extenders affected the post-thaw sperm quality marginally. Whereas osmolalities and glycerol concentrations seemed not to differ, 430 mOsm/kg and 4% glycerol might be preferred. Egg yolk concentrations only differed on sperm velocity (VCL: 84.0 ± 6.7 μm/s in 10% vs. 70.7 ± 6.2 μm/s in 15%, P < 0.05). Our results suggest a good cryotolerance of chamois epididymal spermatozoa, with a preferred extender composition of hyperosmotic buffer, glycerol in the 4% range and lower egg yolk (10% range) than other ruminants.
PB Elsevier
YR 2019
FD 2019-05-13
LK http://hdl.handle.net/10612/10735
UL http://hdl.handle.net/10612/10735
NO Theriogenology, 2019, vol. 125
NO P. 109-114
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 19-abr-2024