RT info:eu-repo/semantics/preprint
T1 Heterologous in vitro fertilization is a good procedure to assess the fertility of thawed ram spermatozoa
A1 García Álvarez, Olga
A1 Maroto Morales, Alejandro
A1 Martínez Pastor, Felipe
A1 Fernández Santos, María Rocío
A1 Esteso, Milagros
A1 Pérez Guzmán, María Dolores
A1 Soler, Ana J.
A2 Biologia Celular
K1 Veterinaria
K1 Heterologous in vitro fertilization
K1 Ram
K1 Fertility
K1 Spermatozoa
K1 Intact-zona oocytes
AB A heterologous in vitro fertilization (IVF) test using calf oocytes with zona pellucida was employed to assess the fertility of thawed ram sperm samples. Six males with significant differences in fertility (P = 0.003) were used. The males were classified as having high fertility (≥42%) and low fertility (≤41%). Male fertility was not influenced by number of inseminated ewes (P = 0.584), insemination technician (P = 0.156), insemination date (P = 0.323) or farm (P = 0.207). Thawed sperm samples were employed to assess several sperm parameters for each male: motility, acrosomal integrity, viability, membrane stability, membrane phospholipid disorder, mitochondrial membrane potential and chromatin stability. These samples were used to carry out a heterologous in vitro fertilization. In vitro-matured calf oocytes (n = 716) were inseminated with thawed ram semen and in vitro cultured for 40 h. Overall, at thawing, variability among males respect to sperm quality was high. Despite this variability, there were not differences (P < 0.05) between fertility groups. Yield of hybrid embryos ranged from 31 to 59% between males. There were not differences between males (P = 0.340). However, there were differences between fertility groups (high fertility: 55%; low fertility: 39%; P = 0.020). Multiple regression analysis showed that the heterologous in vitro fertility was the only predictive parameter for in vivo male fertility. Correlation between both parameters was fair (r2 = 0.760; P = 0.025). These results indicate that heterologous in vitro fertilization tests can be useful to predict the fertility of ram spermatozoa using calf oocytes with intact-zona pellucida.
PB Elsevier
YR 2019
FD 2019-05-13
LK http://hdl.handle.net/10612/10743
UL http://hdl.handle.net/10612/10743
NO Theriogenology, 2009, vol. 71, n. 4
NO P. 643-650
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 26-abr-2024