RT info:eu-repo/semantics/article T1 Quality of frozen-thawed semen in brown bear is not affected by timing of glycerol addition A1 Álvarez Rodríguez, Manuel A1 Álvarez García, Mercedes A1 Gomes Alves, Susana Cláudia A1 Barragán Santos, Santiago A1 Martínez Pastor, Felipe A1 Paz Cabello, Paulino de A1 Anel Rodríguez, Luis A2 Biologia Celular K1 Veterinaria K1 Brown bear K1 Semen K1 Glycerol K1 Freezing AB We have tested several freezing protocols for brown bear semen, modifying the time when glycerol was added (before and after cooling to 5 °C). No differences were found among protocols, indicating a good tolerance of brown bear semen to glycerol. This finding indicates that freezing protocols for brown bear semen could be modified to fit practical solutions which would facilitate preparation of the seminal samples in the field with the addition of glycerol at ambient temperature. PB Elsevier YR 2019 FD 2019-05-13 LK http://hdl.handle.net/10612/10750 UL http://hdl.handle.net/10612/10750 NO Theriogenology, 2011, vol. 75, n. 8 NO P. 1561-1565 DS BULERIA. Repositorio Institucional de la Universidad de León RD 26-abr-2024