RT info:eu-repo/semantics/preprint
T1 Cryobiology of cephalopod (Illex coindetii) spermatophores
A1 Robles Rodríguez, Vanesa
A1 Martínez Pastor, Felipe
A1 Petroni, Giuliano
A1 Fernández Riesco, Marta
A1 Bozzano, Anna
A1 Villanueva, Roger
A2 Biologia Celular
K1 Veterinaria
K1 Mollusca
K1 Spermatophore
K1 Spermatangia
K1 Spermatozoa
K1 Cryopreservation
K1 Flow cytometry
AB Cephalopod culture is expected to increase in the near future and sperm cryopreservation would be a valuable tool to guarantee sperm availability throughout the year and to improve artificial insemination programs. We have studied the tolerance of spermatophores from the oceanic squid Illex coindetii to several cryoprotectants, in two toxicity experiments and a cryopreservation test. Five permeating cryoprotectants were tested: Dimethyl sulfoxide (Me2SO), methanol, glycerol, propylene glycol and ethylene glycol. In the first experiment, spermatophores were exposed to the five cryoprotectants at 5% (v/v) and 15% (v/v) at 4 °C for 5 min. In the second experiment, spermatophores were exposed to the cryoprotectants at 15% using different exposure times: 5, 15 and 30 min. In a third experiment, we tested two cryopreservation protocols: LN2 vapor or −80 °C freezer, using a 15% cryoprotectant and 15 or 30 min of exposure. Viability and mitochondrial activity were assessed using Mitotracker deep red, YOPRO1 and Hoechst 33342, by flow cytometry. Spermatozoa in this species remain viable after cryoprotectant exposure but their quality decreased considerably after cryopreservation, only 5–10% of spermatozoa being motile. Flow cytometry demonstrated that Me2SO may be the most appropriate cryoprotectant for I. coindetii spermatozoa, and shows a first approach on cephalopod sperm cryopreservation, opening new possibilities for the research and culture of this group of molluscs.
PB Elsevier
YR 2019
FD 2019-05-17
LK http://hdl.handle.net/10612/10782
UL http://hdl.handle.net/10612/10782
NO Cryobiology, 2013, vol. 66, n. 3
NO P. 288-294
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 27-abr-2024