RT info:eu-repo/semantics/article T1 Lecithin nanoparticles enhance the cryosurvival of caprine sperm A1 Nadri, T. A1 Towhidi, Armin A1 Zeinoaldini, Saeeid A1 Martínez Pastor, Felipe A1 Mousavi, M. A1 Noei, R. A1 Tar, M. A1 Mohammadi Sangcheshmeh, Abdollah A2 Biologia Celular K1 Veterinaria K1 Nanoparticle K1 Sperm cryopreservation K1 Extender K1 Lecithin K1 Caprine AB This study was designed to compare the effects on goat spermatozoa cryosurvival of nano-lecithin-based (NL), lecithin-based (L) and egg yolk-based (EY) extenders. Ejaculates were collected from four fertile goats using artificial vagina and diluted with nine extenders. NL and L were tested at concentrations 1%, 2%, 3% and 4% (w/v), versus 15% (v/v) egg yolk-based extender. Overall, sperm quality (higher motility, viability and HOST, and lower apoptosis) was higher for NL than for L treatments (P < 0.05 for most cases, except for 1%). NL at 1% and especially at 4% showed lower motility and viability than 2% or 3% NL. NL at 2% achieved a better performance (P < 0.05) than EY for VCL (131.5 ± 1.3 vs. 120.3 ± 1.9 μm/s), VSL (43.9 ± 1.5 vs. 35.8 ± 1.4 μm/s), LIN (35.7 ± 0.6 vs. 29.3 ± 0.8%), WOB (47.0 ± 0.5 vs. 43.9 ± 0.9%) and viability (66.4 ± 1.7 vs. 52.7 ± 1.9%). Late apoptotic spermatozoa were also lower in 2% NL compared to EY (16.0 ± 0.5 vs. 26.3 ± 1.1%, P < 0.001). EY and 2% NL were compared in an IVF trial, with no significant differences in cleavage (68.8 vs. 70.8%) or blastocyst ratios (21.3 vs. 20.8%). In conclusion, using 2% nanolecithin in semen dilution could improve sperm cryosurvival of goat. PB Elsevier YR 2019 FD 2019-05-21 LK http://hdl.handle.net/10612/10809 UL http://hdl.handle.net/10612/10809 NO Theriogenology, 2019, vol. 133 NO P. 38-44 DS BULERIA. Repositorio Institucional de la Universidad de León RD 29-mar-2024