RT info:eu-repo/semantics/preprint
T1 DNA fragmentation assessment by flow cytometry and Sperm–Bos–Halomax (bright‐field microscopy and fluorescence microscopy) in bull sperm
A1 García Macías, Vanesa
A1 Paz Cabello, Paulino de
A1 Martínez Pastor, Felipe
A1 Álvarez García, Mercedes
A1 Gomes Alves, Susana Cláudia
A1 Bernardo, Jana
A1 Anel Rodríguez, Enrique
A1 Anel Rodríguez, Luis
A2 Biologia Celular
K1 Veterinaria
K1 Bull
K1 DNA
K1 Halomax
K1 Sperm chromatin structure assay
K1 Sperm
AB The aim of this study was to find the relationship between fertility (as 90‐day non‐return rates) and DNA fragmentation assessed by two techniques [sperm chromatin structure assay (SCSA) and Sperm–Bos–Halomax (SBH)]. Furthermore, other quality parameters were achieved (motility, morphological abnormalities, cytoplasmic droplets, viability, capacitation and acrosomal and mitochondrial status) and their correlations with fertility were analysed. Bulls were divided into three fertility groups: high [non‐return rate (NRR) ≥ 80], medium (80 < NRR ≥ 70) and low (70 < NRR > 40). The results of this study indicate that there is a good correlation between fertility and different parameters of sperm quality (SBH and SCSA parameters, % of spermatozoa with head, neck and total abnormalities, and % of spermatozoa with proximal cytoplasmic droplets) and differences between fertility groups were observed in some of them (SBH and SCSA parameters and % of spermatozoa with head, neck and total abnormalities). In this sense, SBH parameters rendered good correlations with fertility (r = −0.42 using bright light microscope and r = −0.47 with fluorescence). Also, standard deviation of DNA fragmentation index (SD‐DFI) and DFIh (cells with High DNA fragmentation index) showed good correlations with fertility (r = −0.41 and r = −0.29). No correlations were observed between SCSA and SBH parameters. A multiple regression shows that four parameters (% of proximal cytoplasmic droplets, % of intact acrosomes in total population, SD‐DFI and percentage of fragmented DNA detected by bright light microscope) present a good predictive value of the fertility of sperm samples (r2 = 0.34, p < 0.001)
PB John Wiley & Son
YR 2019
FD 2019-05-22
LK http://hdl.handle.net/10612/10817
UL http://hdl.handle.net/10612/10817
NO International Journal of Andrology, 2007, vol. 30, n. 2
NO P. 88-98
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 26-abr-2024