RT info:eu-repo/semantics/article
T1 Transcriptomics of Haemophilus (Glässerella) parasuis serovar 5 subjected to culture conditions partially mimetic to natural infection for the search of new vaccine antigens
A1 Álvarez Estrada, Álvaro
A1 Gutiérrez Martín, César Bernardo
A1 Rodríguez Ferri, Elías Fernando
A1 Martínez Martínez, Sonia
A2 Sanidad Animal
K1 Sanidad animal
K1 Veterinaria
K1 Haemophilus parasuis
K1 Glässer’s disease
K1 Ganado porcino
K1 Vacunas
AB Haemophilus (Glässerella) parasuis is the etiological agent of Glässer’s disease in pigs. Control of this disorder has been traditionally based on bacterins. The search for alternative vaccines has focused mainly on the study of outer membrane proteins. This study investigates the transcriptome of H. (G.) parasuis serovar 5 subjected to in vitro conditions mimicking to those existing during an infection (high temperature and iron-restriction), with the aim of detecting the overexpression of genes coding proteins exposed on bacterial surface, which could represent good targets as vaccine candidates.The transcriptomic approach identified 13 upregulated genes coding surface proteins: TbpA, TbpB, HxuA, HxuB, HxuC, FhuA, FimD, TolC, an autotransporter, a protein with immunoglobulin folding domains, another large protein with a tetratricopeptide repeat and two small proteins that did not contain any known domains. Of these, the first six genes coded proteins being related to iron extraction.Six of the proteins have already been tested as vaccine antigens in murine and/or porcine infection models and showed protection against H. (G.) parasuis. However, the remaining seven have not yet been tested and, consequently, they could become useful as putative antigens in the prevention of Glässer’s disease. Anyway, the expression of this seven novel vaccine candidates should be shown in other serovars different from serovar 5.
PB Springer
YR 2019
FD 2019-05-27
LK http://hdl.handle.net/10612/10840
UL http://hdl.handle.net/10612/10840
NO BMC Veterinary Research, 2018, vol. 14 (1), Nov 6
NO 11 p.
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 23-abr-2024