RT info:eu-repo/semantics/article
T1 Characterisation, antimicrobial resistance and diversity of atypical EPEC and STEC isolated from COW’S milk, cheese and dairy cattle farm environments
A1 Rios, Edson Antonio
A1 Santos Buelga, Jesús Ángel
A1 García-Meniño, Isidro
A1 Flament-Simon, Saskia C.
A1 Blanco, Jorge
A1 García López, María Luisa
A1 Otero Carballeira, Andrés
A1 Rodríguez Calleja, José María
A2 Nutricion y Bromatologia
K1 Tecnología de los alimentos
K1 Multidrug resistance
K1 Serotypes
K1 Diarrheagenic E. coli
K1 E. albertii
K1 aEPEC
AB This study was carried out to determine the occurrence and characteristics of enteropathogenic18 Escherichia coli (EPEC) and Shiga toxin-producing E. coli (STEC) strains in cow’s milk, cheese19 and dairy cattle farm environments, and to estimate distribution of antimicrobial resistance. A20 collection of 18 atypical EPEC -aEPEC, 14 STEC, and one E. albertii was obtained and21 characterized from 502 samples. Occurrence of aEPEC in cow’s milk was high (>6%) whereas non-22 O157 STEC was isolated in ca. 2% of milk samples. Detection of these diarrheagenic E. coli was absent in more than 100 cheese samples obtained from raw milk. This is the first 23 report identifying24 E. albertii (O69:HNM) in a dairy cattle farm. Nearly one-third of aEPEC strains showed25 antimicrobial resistance, mostly presenting a multidrug resistance pattern. One clonal complex26 (ST20 Cplx) containing aEPEC strains from milk and faecal samples was determined. Two STEC27 strains belonged to serotypes with importance in human disease (O91:H21 and O55:H8) and were28 isolated from air samples which suggests a high dissemination potential. Spanish bulk tank cow’s29 milk can constitute an important source of aEPEC strains besides STEC, bearing multiple30 antimicrobial resistance and with high diversity of both serotypes and genetic features linked to31 potential human infection
PB Elsevier
YR 2019
FD 2019-07-03
LK http://hdl.handle.net/10612/10995
UL http://hdl.handle.net/10612/10995
NO LWT - Food Science and Technology
NO 25 p.
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 25-abr-2024