RT info:eu-repo/semantics/article
T1 Systemic and local immune responses  in sheep after Neospora caninum experim infection at early, mid and late gestation
A1 Arranz Solis, David
A1 Benavides Silván, Julio
A1 Regidor Cerrillo, Javier
A1 Horcajo Iglesias, María del Pilar
A1 Castaño Labajo, Pablo
A1 Ferreras Estrada, María Del Carmen
A1 Jiménez Pelayo, Laura
A1 Collantes Fernández, Esther
A1 Ferré Pérez, Ignacio
A1 Hemphill, Andrew
A1 Pérez Pérez, Valentín
A1 Ortega Mora, Luis Miguel
A2 Sanidad Animal
K1 Inmunología
K1 Veterinaria
K1 Ganado ovino
K1 Embarazo
K1 Inmunología veterinaria
K1 Neospora caninum
K1 2401.11 Patología Animal
AB Besides its importance in cattle, Neospora caninum may also pose a high risk as abortifacient for small ruminants. We have recently demonstrated that the outcome of experimental infection of pregnant sheep with 106 Nc-Spain7 tachyzoites is strongly dependent on the time of gestation. In the current study, we assessed peripheral and local immune response in those animals. Serological analysis revealed earlier and higher IFN-γ and IgG responses in ewes infected at early (G1) and mid (G2) gestation, when abortion occurred. IL-4 was not detected in sera from any sheep. Inflammatory infiltrates in the placenta mainly consisted of CD8+ and, to a lesser extent, CD4+ T cells and macrophages (CD163+). The infiltrate was more intense in sheep infected at mid-gestation. In the foetal mesenchyme, mostly free tachyzoites were found in animals infected at G1, while those infected in G2 displayed predominantly particulate antigen, and parasitophorous vacuoles were detected in sheep infected at G3. A similar pattern of placental cytokine mRNA expression was found in all groups, displaying a strengthened upregulation of IFN-γ and IL-4 and milder increases of TNF-α and IL-10, reminiscent of a mixed Th1 and Th2 response. IL-12 and IL-6 were only slightly upregulated in G2, and TGF-β was downregulated in G1 and G2, suggestive of limited T regulatory (Treg) cell activity. No significant expression of TLR2 or TLR4 could be detected. In summary, this study confirms the pivotal role of systemic and local immune responses at different times of gestation during N. caninum infection in sheep.
PB Springer
SN 0928-4249
LK http://hdl.handle.net/10612/12291
UL http://hdl.handle.net/10612/12291
NO P. 1-13
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 25-abr-2024