RT info:eu-repo/semantics/article T1 Behaviour of Non-O157 STEC and Atypical EPEC during the Manufacturing and Ripening of Raw Milk Cheese A1 Rios, Edson Antonio A1 Ramos Pereira, Juliana A1 Santos Buelga, Jesús Ángel A1 López Díaz, Teresa María A1 Otero Carballeira, Andrés A1 Rodríguez Calleja, José María A2 Tecnologia de los Alimentos K1 Tecnología de los alimentos K1 Raw Milk Cheese K1 Non-O157 STEC K1 Atypical EPEC K1 Diarrhoeagenic E. coli K1 Cheese processing AB This study was carried out to assess the survival of Shiga toxin-producing E. coli (STEC) and atypical enteropathogenic Escherichia coli (aEPEC) during the traditional manufacturing and ripening of Spanish hard cheese from raw cow’s milk. Milk samples were spiked with up to 3.1–3.5 log cfu/mL of one strain of STEC (O140:H32 serotype) and one of aEPEC (serotype O25:H2). The first steps of cheesemaking allow for a STEC and aEPEC increase of more than 1 log cfu/mL (up to 4.74 log cfu/g and 4.55 log cfu/g, respectively). After cheese pressing, a steady reduction of both populations was observed, with the STEC strain being more sensitive. The studied pathogenic E. coli populations decreased by 1.32 log cfu/g in STEC and 0.59 log cfu/g in aEPEC in cheese ripened during a minimum period of 60 d. Therefore, a moderate contamination by these diarrhoeagenic E.coli pathotypes, in particular, with aEPEC, on cheese manufactured from raw milk may not be totally controlled through the cheesemaking process and during a maturation of 90 d. These findings remark the importance of improvement in bacteriological quality of raw milk and crosscontamination prevention with diarrhoeagenic E. coli in the dairy industry PB MDPI LK http://hdl.handle.net/10612/12369 UL http://hdl.handle.net/10612/12369 NO 12 p. DS BULERIA. Repositorio Institucional de la Universidad de León RD 19-abr-2024