RT info:eu-repo/semantics/article T1 The Milk Microbiota of the Spanish Churra Sheep Breed: New Insights into the Complexity of the Milk Microbiome of Dairy Species A1 Esteban Blanco, Cristina A1 Gutiérrez Gil, Beatriz A1 Marina García, Héctor A1 Pelayo, Rocío A1 Suárez Vega, Aroa A1 Acedo, Alberto A1 Arranz Santos, Juan José A2 Algebra K1 Genética K1 Veterinaria K1 Assaf; Churra; dairy sheep; milk microbiota; 16S rRNA gene sequencing AB Milk from healthy animals has classically been considered a sterile fluid. With the development of massively parallel sequencing and its application to the study of the microbiome of different body fluids, milk microbiota has been documented in several animal species. In this study, the main objective of this work was to access bacterial profiles of healthy milk samples using the next-generation sequencing of amplicons from the 16S rRNA gene to characterise the milk microbiome of the Churra breed. A total of 212 samples were collected from two Churra dairy farms with a different management system. The core milk microbiota in Churra ewes includes lesser genera (only two taxa: Staphylococcus and Escherichia/Shigella) than studies reported in other dairy species or even in a previous study in Assaf sheep milk. We found that diversity values in the two flocks of Churra breed were lower than the diversity of the milk microbiota in Assaf. The non-metric multidimensional scaling (NMDS) ordination using Bray-Curtis distance separates samples based on their microbiota composition. The information reported here might be used to understand the complex issue of milk microbiota composition. LK http://hdl.handle.net/10612/13488 UL http://hdl.handle.net/10612/13488 NO Artículo de Investigación científica DS BULERIA. Repositorio Institucional de la Universidad de León RD 29-mar-2024