RT info:eu-repo/semantics/article
T1 Microbial colonization and resistome dynamics in food processing environments of a newly opened pork cutting industry during 1.5 years of activity
A1 Cobo-Díaz, José F.
A1 Álvarez Molina, Adrián
A1 Alexa, Elena A.
A1 Walsh, Calum J.
A1 Mencía-Ares, Oscar
A1 Puente-Gómez, Paula
A1 Likotrafiti, Eleni
A1 Fernández-Gómez, Paula
A1 Prieto, Bernardo
A1 Crispie, Fiona
A1 Ruiz, Lorena
A1 González Raurich, María Montserrat
A1 López, Mercedes
A1 Prieto Maradona, Miguel
A1 Cotter, Paul
A1 Álvarez Ordóñez, Avelino
A2 Microbiologia
K1 Genética
K1 Tecnología de los alimentos
K1 Metagenomics
K1 Food processing environments
K1 Antimicrobial resistance
K1 Microbial ecology
AB Background: The microorganisms that inhabit food processing environments (FPE) can strongly influence theassociated food quality and safety. In particular, the possibility that FPE may act as a reservoir of antibiotic-resistantmicroorganisms, and a hotspot for the transmission of antibiotic resistance genes (ARGs) is a concern in meatprocessing plants. Here, we monitor microbial succession and resistome dynamics relating to FPE through adetailed analysis of a newly opened pork cutting plant over 1.5 years of activity.Results: We identified a relatively restricted principal microbiota dominated by Pseudomonas during the first 2months, while a higher taxonomic diversity, an increased representation of other taxa (e.g., Acinetobacter,Psychrobacter), and a certain degree of microbiome specialization on different surfaces was recorded later on. Anincrease in total abundance, alpha diversity, and β-dispersion of ARGs, which were predominantly assigned toAcinetobacter and associated with resistance to certain antimicrobials frequently used on pig farms of the region,was detected over time. Moreover, a sharp increase in the occurrence of extended-spectrum β-lactamase-producing Enterobacteriaceae and vancomycin-resistant Enterococcaceae was observed when cutting activitiesstarted. ARGs associated with resistance to β-lactams, tetracyclines, aminoglycosides, and sulphonamides frequentlyco-occurred, and mobile genetic elements (i.e., plasmids, integrons) and lateral gene transfer events were mainlydetected at the later sampling times in drains.Conclusions: The observations made suggest that pig carcasses were a source of resistant bacteria that thencolonized FPE and that drains, together with some food-contact surfaces, such as equipment and table surfaces,represented a reservoir for the spread of ARGs in the meat processing facility.
PB BMC
LK http://hdl.handle.net/10612/13494
UL http://hdl.handle.net/10612/13494
NO Artículo versión publicada
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 24-abr-2024