RT info:eu-repo/semantics/article
T1 Extracellular vesicle and soluble fractions of adipose tissue-derived mesenchymal stem cells secretome induce inflammatory cytokines modulation in an in vitro model of discogenic pain
A1 González-Cubero, Elsa
A1 González-Fernández, María L.
A1 Olivera, Elias R.
A1 Villar Suárez, María Vega
A2 Anatomia y Anatomia Patologicas Comparadas
K1 Biología
K1 Adipose tissue mesenchymal stem cells
K1 Secretome
K1 Extracellular vesicles
K1 Soluble fraction
K1 Inflammation
K1 Cytokines
K1 Intervertebral disc
K1 2407 Biología Celular
K1 3109.01 Anatomía
AB BACKGROUND CONTEXT: Mesenchymal Stem Cells (MSCs) secretome or conditionedmedium (CM) is a complex cocktail of different molecules, some of which, particularly those con-tained in extracellular vesicles, already have proven therapeutic applications.PURPOSE: CM may well represent promising therapy for discogenic pain and the intention of thiswork is to assess its therapeutic potential using an in vitro model of this condition.STUDY DESIGN: This is an experimental study.METHODS: Our in vitro model comprised Nucleus Pulposus (NP) and Annulus Fibrosus (AF)cells inflamed with TNF. To assess the potential therapeutic value of CM and its components,extracellular vesicles (EVs) and soluble culture fraction (SF), cell inflammation took place under 3different conditions: either in the presence of whole CM, isolated EVs or SF, and concentrations ofpro-inflammatory cytokines, metalloproteinases (MMPs) and neurotrophic factors produced in all3 cases were compared.RESULTS: In the presence of whole CM, both in vitro gene expression by the NP and AF test cellsand analysis of their protein content showed high modulatory effects on inflammation and MMPinhibition. The presence of EVs and SF showed similar but much smaller effects, and this was par-ticularly marked in the case of NP cells.CONCLUSIONS: Our results show that, compared to EVs and SF, the presence of whole CM hasthe greatest positive effect on the modulation of pro-inflammatory and catabolic factors. Theseobservations suggest that CM could protect against inflammation and the resulting intervertebraldisc (IVD) degeneration that leads to discogenic pain.CLINICAL SIGNIFICANCE: Many patients' expectations are not met by current non-operativeand surgical treatments for discogenic low back pain. We propose the use of the MSCs secretome
PB Elsevier
SN 1529-9430
LK http://hdl.handle.net/10612/14520
UL http://hdl.handle.net/10612/14520
NO .
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 24-abr-2024