RT info:eu-repo/semantics/article
T1 Cold-Shock Test Is a Practical Method for Selecting Boar Ejaculates Yielding Appropriate Seminal Plasma for Post-Thawing Supplementation
A1 Lacalle Fernández, Estíbaliz
A1 Núñez, Andrea
A1 Fernández Alegre, Estela
A1 Crespo-Félez, Itxaso
A1 Domínguez Fernández de Tejerina, Juan Carlos
A1 Alonso de la Varga, Marta Elena
A1 González Urdiales, Raúl
A1 Martínez Pastor, Felipe
A2 Bioquimica y Biologia Molecular
K1 Biología
K1 Boar
K1 Seminal plasma
K1 Cryopreserved semen
K1 Cold-shock test
K1 Data clustering
K1 Sperm motility
K1 Sperm physiology
K1 Chromatin status
K1 Osmotic resistance test
K1 DNA fragmentation
AB Artificial insemination (AI) with cryopreserved semen is still unreliable for extensive pig industry application. Adding seminal plasma (SP) could improve post-thawing quality, but its suitability could vary. We applied a simple cold-shock test (CST, 5 min at 0 °C) on neat semen for classifying ejaculates (n = 63) as resistant or sensitive, obtaining two SP pools (CST-resistant: SPr, sensitive: SPs). Subsequently, frozen/thawed spermatozoa from six boars were incubated (37 °C) in MR-A® extender (control), 20% SPr, or 20% SPs, and analyzed at 0, 2, and 4 h. SP improved total and progressive motility, with a higher effect for SPr and STR (p < 0.05), decreasing kinematic parameters VCL and VAP, ALH, and BCF. Sperm viability was unaffected. SP increased apoptotic and membrane disorder ratios, and acrosomal damage, not affecting the chromatin structure (DNA fragmentation and immaturity by SCSA), protamination (CMA3), or disulfide levels (mBBr). However, the proportion of spermatozoa with elevated free thiols (disulfide bridges reduction) significantly increased. Results support a stimulatory role of SP on thawed semen, with additional benefits from SPr. The effect of SP and especially SPr after AI should be tested since CST could be a practical test for selecting suitable ejaculates in AI centers.
PB MDPI
LK http://hdl.handle.net/10612/14793
UL http://hdl.handle.net/10612/14793
NO .
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 18-may-2024