RT info:eu-repo/semantics/article
T1 Melatonin affects red deer spermatozoa motility and physiology in capacitating and non‐capacitating conditions
A1 Fernández‐Alegre, Estela
A1 Lacalle Fernández, Estíbaliz
A1 Soriano Úbeda, Cristina de las Mercedes
A1 Domínguez Fernández de Tejerina, Juan Carlos
A1 Casao, Adriana
A1 Martínez Pastor, Felipe
A2 Biologia Celular
K1 Biología
K1 Melatonin
K1 Red deer
K1 Sperm capacitation
K1 Sperm physiology
K1 Spermatozoon
AB [EN] Melatonin affects sperm physiology, possibly through membrane receptors. Effects were tested at low concentrations (1 pM, 100 pM, 10 nM and 1 µM) in red deer epididymal spermatozoa as a model for high-seasonality species. Samples were incubated with melatonin as uncapacitated or capacitating conditions (heparin) and evaluated for motility and physiology (flow cytometry). Most effects occurred at low concentrations (nM-pM), mainly protecting from apoptosis and maintaining acrosomal integrity, suggesting a role for membrane receptors rather than a direct antioxidant effect. Intracellular calcium was not affected, differing from other studies and perhaps because of the epididymal origin. This study supports the relevance of melatonin on sperm physiology and could contribute to the application of reproductive technologies in wild ruminants.
PB Wiley-Blackwell
SN 0936-6768
LK http://hdl.handle.net/10612/15380
UL http://hdl.handle.net/10612/15380
NO Fernández-Alegre, E., Lacalle, E., Soriano-Úbeda, C., Carlos Domínguez, J., Casao, A., & Martínez-Pastor, F. (2022). Melatonin affects red deer spermatozoa motility and physiology in capacitating and non-capacitating conditions. Reproduction in Domestic Animals, 57(S5), 82-85. https://doi.org/10.1111/RDA.14137
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 25-abr-2024