RT info:eu-repo/semantics/article
T1 Extension of the equilibration period up to 24 h maintains the post-thawing quality of Holstein bull semen frozen with OPTIXcell®
A1 Salman, Amer
A1 Fernández-Alegre, Estela
A1 Francisco-Vázquez, Rubén
A1 Gómez-Martín, Rubén
A1 Fernández-Fernández, Alejandro
A1 Areán-Dablanca, Héctor
A1 Domínguez Fernández de Tejerina, Juan Carlos
A1 González Montaña, José Ramiro
A1 Caamaño Gualdoni, José Néstor
A1 Martínez Pastor, Felipe
A2 Biologia Celular
K1 Biología
K1 Bull
K1 OPTIXcell
K1 Equilibration time
K1 Sperm cryopreservation
K1 Sperm quality
K1 Fertility
AB [EN] Semen cryopreservation in bovine livestock is well established, but logistics often require deviations from standard protocols. Extending the equilibration time to the following day is convenient in many situations. To improve our knowledge of the effects of this modification, we studied the post-thawing and post-incubation (4 h, 38 °C) sperm quality after freezing with 4 or 24-h extension in the OPTIXcell extender by using an ample panel of analyses: CASA for motility; flow cytometry for viability, physiology, oxidative stress, and chromatin parameters (DNA fragmentation, chromatin compaction, and thiol groups status); and spectrometry for malondialdehyde production. Semen was obtained from 12 Holstein bulls. The 24-h equilibration time showed few significant effects, with only a tiny decrease in progressive motility and a positive impact on chromatin structure. The incubation removed some of these effects, with the pattern for chromatin compaction remaining the same. No detrimental oxidative stress or increase in apoptotic or capacitation markers was detected. Additionally, the individual bull interacted with the effects of the incubation and the equilibration, especially regarding the chromatin status. Whereas this interaction did not critically affect sperm quality, it could be relevant in practice. Bull fertility as non-return rates (NRR56) was associated with some sperm parameters (especially with an improved chromatin structure) but not in the 4-h post-thawing analysis. Our study supports that extending the equilibration time by at least 24-h is feasible for bull semen freezing with the OPTIXcell extender.
PB Elsevier
SN 0378-4320
LK http://hdl.handle.net/10612/15843
UL http://hdl.handle.net/10612/15843
NO Salman, A., Fernández-Alegre, E., Francisco-Vázquez, R., Gómez-Martín, R., Fernández-Fernández, A., Areán-Dablanca, H., Domínguez, J. C., González-Montaña, J. R., Caamaño, J. N., & Martínez-Pastor, F. (2023). Extension of the equilibration period up to 24 h maintains the post-thawing quality of Holstein bull semen frozen with OPTIXcell®. Animal Reproduction Science, 250(107209), 107209. https://doi.org/10.1016/j.anireprosci.2023.107209
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 25-abr-2024