RT info:eu-repo/semantics/article
T1 Ovine fertility by artificial insemination in the breeding season could be affected by intraseasonal variations in ram sperm proteomic profile
A1 Neila-Montero, Marta
A1 Álvarez García, Mercedes
A1 Fernández Riesco, Marta
A1 Montes-Garrido, Rafael
A1 Palacin-Martinez, Cristina
A1 Silva-Rodríguez, Antonio
A1 Martín-Cano, Francisco E.
A1 Peña, Fernando J.
A1 Paz Cabello, Paulino de
A1 Anel Rodríguez, Luis
A1 Anel López, Luis
A2 Anatomia y Anatomia Patologicas Comparadas
K1 Anatomía
K1 Cervical insemination
K1 Cooled semen
K1 Proteomic analysis
K1 Seasonality
K1 Sheep
K1 Sperm proteome
K1 3109.01 Anatomía
AB [EN] It is important to note that seasonality could affect ram reproductive parameters, and therefore, fertility results after artificial insemination. In this work, 1) we assessed fertility rates after cervical artificial insemination of 11,805 ewes at the beginning (June 21st to July 20th) and at the end (November 20th to December 21st) of the reproductive season in the Assaf breed for the last four years, and 2) we aimed to identify male factors influencing the different reproductive success obtained depending on the time at the mating season in which ovine artificial insemination was performed. For this purpose, we evaluated certain ram reproductive and ultrasonographical parameters as well as we performed a multiparametric and proteomic sperm analysis of 6–19 rams at two very distant points in the mating season (July as Early Breeding Season –EBS– and November as Late Breeding Season –LBS–). Rutinary assessments carried out in the ovine reproduction centers (testicular volume, libido, sperm production and mass motility) showed non-significant differences (P ≥ 0.05) between both studied times, as well as the ram ultrasonographic evaluation (Resistive and Pulsatility Index as Doppler parameters; and pixels mean gray level, and hypoechoic areas percentage and density as echotexture parameters). However, at level of sperm functionality, although sperm quality appeared non-significantly lower (P ≥ 0.05) in the EBS, we identified a significantly different (P < 0.05) sperm proteomic profile between the seasonality points. The following proteins were identified with the lowest abundance in the EBS with a fold change > 4, a P = 2.40e-07, and a q = 2.23e-06: Fibrous Sheath-Interacting Protein 2, Disintegrin and Metalloproteinase Domain-Containing Protein 20-like, Phosphoinositide-Specific Phospholipase C, Tektin 5, Armadillo Repeat-Containing Protein 12 Isoform X3, Solute Carrier Family 9B1, Radial Spoke Head Protein 3 Homolog, Pro-Interleukin-16, NADH Dehydrogenase [Ubiquinone] 1 Alpha Subcomplex Subunit 8, Testis, Prostate and Placenta-Expressed Protein, and Acyl Carrier Protein Mitochondrial. In conclusion, while our basic analyses on male and sperm quality showed similar results between the beginning and the end of the breeding season, on a proteomic level we detected a lower expression of sperm proteins linked to the energy metabolism, sperm-oocyte interactions, and flagellum structure in the EBS. Probably, this different protein expression could be related to the lower fertility rate of Assaf ewes after cervical artificial insemination at this time. More importantly, sperm proteins can be used as highly effective molecular markers in predicting sperm fertilization ability related to intraseasonal variations.
PB Elsevier
SN 0093-691X
LK http://hdl.handle.net/10612/16787
UL http://hdl.handle.net/10612/16787
NO Neila-Montero, M., Alvarez, M., Riesco, M. F., Montes-Garrido, R., Palacin-Martinez, C., Silva-Rodríguez, A., Martín-Cano, F. E., Peña, F. J., de Paz, P., Anel, L., y  Anel-Lopez, L. (2023). Ovine fertility by artificial insemination in the breeding season could be affected by intraseasonal variations in ram sperm proteomic profile. Theriogenology, 208, 28–42. https://doi.org/10.1016/j.theriogenology.2023.05.030
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 22-may-2024