RT info:eu-repo/semantics/article
T1 Altered tissue distribution of flaxseed lignans and their metabolites in Abcg2 knockout mice
A1 García-Mateos, Dafne
A1 García-Villalba, Rocío
A1 Otero, Jon Andoni
A1 Marañón, José Ángel
A1 Espín, Juan Carlos
A1 Álvarez, Ana I.
A1 Merino Peláez, Gracia
A2 Fisiologia
K1 Fisiología
K1 Cáncer
K1 BCRP/ABCG2
K1 Proteínas
AB Lignans are dietary polyphenols, which are metabolized by gut microbiota into the phytoestrogenicmetabolites enterolignans, mainly enterolactone and enterodiol. Breast Cancer Resistance Protein(BCRP/ABCG2) is an efflux transporter that affects the plasma and milk secretion of several drugs andnatural compounds. We hypothesized here that Abcg2 could influence the levels of lignans and theirderived metabolites in target tissues. Consequently, we aimed to evaluate the role of Abcg2 in thetissue distribution of these compounds. We used Abcg2−/− knockout and wild-type male mice fed witha lignan-enriched diet for one week and analysed their plasma, small intestine, colon, liver, kidneys andtesticles. High levels of lignans as well as enterolignans and their glucuronide and sulfate conjugates inthe small intestine and colon were detected, with higher concentrations of the conjugates in the wildtype compared with Abcg2−/− mice. Particularly relevant was the detection of 24-fold and 8-foldhigher concentrations of enterolactone-sulfate and enterolactone-glucuronide, respectively, in thekidney of Abcg2−/− compared with wild-type mice. In conclusion, our study showed that lignans andtheir derived metabolites were in vivo substrates of Abcg2, which affected their plasma and tissuelevels. These results highlight the role of Abcg2 in influencing the health-beneficial properties ofdietary lignans.
PB Royal Society of Chemistry
SN 2042-6496
LK https://hdl.handle.net/10612/17442
UL https://hdl.handle.net/10612/17442
NO García-Mateos, D., García-Villalba, R., Otero, J. A., Marañón, J. A., Espín, J. C., Álvarez, A. I., & Merino, G. (2018). An altered tissue distribution of flaxseed lignans and their metabolites in Abcg2 knockout mice. Food and Function.
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 21-may-2024