RT info:eu-repo/semantics/article
T1 Characterization and expression of the arginine biosynthesis gene cluster of Streptomyces clavuligerus
A1 Rodríguez García, Antonio
A1 Fuente, Álvaro de la
A1 Pérez Redondo, Rosario
A1 Martín Martín, Juan Francisco
A1 Liras Padín, Paloma
A2 Microbiologia
K1 Biotecnología
K1 Streptomyces clavuligerus
K1 S1 nuclease assay
K1 Northern hybridization
K1 N-acetylglutamate synthetase
K1 N-acetylornithine aminotransferase
K1 N-acetylornithinase
K1 Arginine biosynthesis
K1 argCJBDRGH
K1 Ornithine N-acetyltransferase
K1 2414.03 Metabolismo Bacteriano
K1 2415.01 Biología Molecular de Microorganismos
AB [EN] A cluster of genes argCJBDRGH containing most of the arginine biosynthesis genes has been found in Streptomyces clavuligerus after sequencing a 8.3 kb DNA region containing overlapping sequences of two DNA fragments known to contain arginine biosynthesis genes. Subcloning, complementation of E. coli arginine auxotrophic strains and enzymatic assays confirmed the identity of each gene. S1 nuclease mapping studies and Northern hybridization analysis revealed the formation of two large transcripts corresponding to argCJBDR and argGH. The amount of each of these mRNAs is 10 to 44 times higher in a S. clavuligerus argR-disrupted mutant than in the wild type confirming the existence of an ArgR-mediated control of arginine biosynthesis gene expression. A low level constitutive monocistronic transcript of argR was observed in S. clavuligerus cells. Most of the argGH transcript initiating at an adenine 29 nt upstream of the argG initiation codon appears to stop at a termination stem and loop structure present downstream of the argG gene
PB Karger
SN 1464-1801
LK https://hdl.handle.net/10612/17708
UL https://hdl.handle.net/10612/17708
NO Rodríguez-García, A., De La Fuente, A., Pérez-Redondo, R., Martín, J. F. & Liras, P. (2000). Characterization and expression of the arginine biosynthesis gene cluster of Streptomyces clavuligerus. Journal of Molecular Microbiology and Biotechnology, 2(4), 543-550
NO Revista continuada por Microbial Physiology
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 21-may-2024