RT info:eu-repo/semantics/article
T1 A cephalosporin C acetylhydrolase is present in the cultures of Nocardia lactamdurans
A1 Cardoza, Rosa E.
A1 Velasco, Javier
A1 Martín Martín, Juan Francisco
A1 Liras Padín, Paloma
A2 Microbiologia
K1 Biología
K1 Cephalosporin C acetylhydrolase
K1 Nocardia lactamdurans
K1 Deacetylcephalosporin c
K1 Beta-lactam antibiotics
K1 2414 Microbiología
K1 2414.01 Antibióticos
K1 2414.07 Metabolismo Microbiano
K1 2403 Bioquímica
AB [EN] Two protein bands with strong esterase activity are present in broths of Nocardia lactamdurans MA4213 cultures. One of them shows cephalosporin C acetylhydrolase (CAH) activity. This activity is maximal at 48 h of growth and shows a pattern of regulation slightly different from that of cephamycin production in medium supplemented with glucose (166 mM), glycerol (326 mM) or ammonium chloride (60 mM). The CAH activity was purified to homogeneity by DEAE-Sepharose ion-exchange, Sephadex G-75 gel filtration, and phenyl-Sepharose hydrophobic interaction chromatography. It showed a molecular mass of 72,100 Da. The N-terminus of the protein was determined and showed the amino acid sequence GGAAPGGPGAHPLWLPAGKD. The enzyme showed K(m) values of 7.0 mM and 8.3 mM for cephalosporin C and 7-aminocephalosporanic acid respectively but was not active on cephamycin C.
PB Springer Link
SN 0175-7598
LK https://hdl.handle.net/10612/17798
UL https://hdl.handle.net/10612/17798
NO Cardoza, R. E., Velasco, J., Martín, J. F. M., & Liras, P. (2000). A cephalosporin C acetylhydrolase is present in the cultures of Nocardia lactamdurans. Applied Microbiology and Biotechnology, 54(3), 406-412. https://doi.org/10.1007/s002530000391
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 13-may-2024