RT info:eu-repo/semantics/article
T1 Targeted Inactivation of the mecB Gene, Encoding Cystathionine-γ-Lyase, Shows that the Reverse Transsulfuration Pathway Is Required for High-Level Cephalosporin Biosynthesis inAcremonium chrysogenum C10 but Not for Methionine Induction of the Cephalosporin Genes
A1 Liu, Gang
A1 Casqueiro Blanco, Francisco Javier
A1 Bañuelos, Óscar
A1 Cardoza, Rosa E.
A1 Gutiérrez Martín, Santiago
A1 Martín Martín, Juan Francisco
A2 Microbiologia
K1 Biología
K1 Filamentous fungi
K1 Beta-lactam antibiotics
K1 Cephalosporin biosynthesis
K1 Cystathionine-γ-Lyase
K1 2414 Microbiología
K1 2414.01 Antibióticos
K1 2414.06 Hongos
K1 2414.07 Metabolismo Microbiano
K1 2415.01 Biología Molecular de Microorganismos
AB [EN] Targeted gene disruption efficiency in Acremonium chrysogenum was increased 10-fold by applying the double-marker enrichment technique to this filamentous fungus. Disruption of themecB gene by the double-marker technique was achieved in 5% of the transformants screened. Mutants T6 and T24, obtained by gene replacement, showed an inactive mecB gene by Southern blot analysis and no cystathionine-γ-lyase activity. These mutants exhibited lower cephalosporin production than that of the control strain, A. chrysogenum C10, in MDFA medium supplemented with methionine. However, there was no difference in cephalosporin production between parental strain A. chrysogenum C10 and the mutants T6 and T24 in Shen's defined fermentation medium (MDFA) without methionine. These results indicate that the supply of cysteine through the transsulfuration pathway is required for high-level cephalosporin biosynthesis but not for low-level production of this antibiotic in methionine-unsupplemented medium. Therefore, cysteine for cephalosporin biosynthesis in A. chrysogenum derives from the autotrophic (SH2) and the reverse transsulfuration pathways. Levels of methionine induction of the cephalosporin biosynthesis gene pcbC were identical in the parental strain and the mecB mutants, indicating that the induction effect is not mediated by cystathionine-γ-lyase.
PB American Society for Microbiology
SN 0021-9193
LK https://hdl.handle.net/10612/17811
UL https://hdl.handle.net/10612/17811
NO Liu, G., Casqueiro, J., Bañuelos, Ó., Cardoza, R. E., Gutiérrez, S., y Martı́n, J. F. (2001). Targeted Inactivation of the mecB Gene, Encoding Cystathionine-γ-Lyase, Shows that the Reverse Transsulfuration Pathway Is Required for High-Level Cephalosporin Biosynthesis in Acremonium chrysogenum C10 but Not for Methionine Induction of the Cephalosporin Genes. Journal of Bacteriology, 183(5), 1765-1772. https://doi.org/10.1128/jb.183.5.1765-1772.2001
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 15-may-2024